Identification of Cell Surface Antigen Expression in Canine Hepatocellular Carcinoma Cell Lines

Ayumi FUJIMOTO; Sakurako NEO; Chinatsu ISHIZUKA; Takashi KATO; Kazuhito SEGAWA; Shinpei KAWARAI; Kikumi OGIHARA; Masaharu HISASUE; Ryo TSUCHIYA

doi:10.1292/jvms.12-0549

Clinical Pathology

Identification of Cell Surface Antigen Expression in Canine Hepatocellular Carcinoma Cell Lines

Ayumi FUJIMOTO, Sakurako NEO, Chinatsu ISHIZUKA, Takashi KATO, Kazuhito SEGAWA, Shinpei KAWARAI, Kikumi OGIHARA, Masaharu HISASUE, Ryo TSUCHIYA

Author information

Ayumi FUJIMOTO
Laboratory of Veterinary Internal Medicine II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoku, Sagamihara, Kanagawa 252–5201, Japan
Sakurako NEO
Laboratory of Veterinary Internal Medicine II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoku, Sagamihara, Kanagawa 252–5201, Japan
Chinatsu ISHIZUKA
Laboratory of Veterinary Internal Medicine II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoku, Sagamihara, Kanagawa 252–5201, Japan
Takashi KATO
Laboratory of Veterinary Internal Medicine II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoku, Sagamihara, Kanagawa 252–5201, Japan
Kazuhito SEGAWA
Laboratory of Veterinary Internal Medicine II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoku, Sagamihara, Kanagawa 252–5201, Japan
Shinpei KAWARAI
Department of Veterinary Teaching Hospital, School of Life and Environmental Science, Azabu University, 1–17–71 Fuchinobe, Chuoku, Sagamihara, Kanagawa 252–5201, Japan
Kikumi OGIHARA
Laboratory of Pathology, School of Life and Environmental Science, Azabu University, 1–17–71 Fuchinobe, Chuoku, Sagamihara, Kanagawa 252–5201, Japan
Masaharu HISASUE
Laboratory of Veterinary Internal Medicine II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoku, Sagamihara, Kanagawa 252–5201, Japan
Ryo TSUCHIYA
Laboratory of Veterinary Internal Medicine II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoku, Sagamihara, Kanagawa 252–5201, Japan

Keywords: canine, hepatocellular carcinoma, liver, stem cell, tumor marker

JOURNAL FREE ACCESS

2013 Volume 75 Issue 6 Pages 831-835

DOI https://doi.org/10.1292/jvms.12-0549

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Abstract

The characteristics of surface antigens in canine hepatocellular carcinoma (cHCC) have not been clarified. The objective of this study was to investigate surface antigens, which are considered as stem/progenitor or cancer cell markers, in cHCC cell lines. Expression of various antigens including CD29, CD34, CD44, CD90, CD133 and Dlk-1 was assessed in four cHCC cell lines by flow cytometry. CD44, CD133 and Dlk-1 expression was detectable in all cell lines, and three cell lines expressed CD29. These results indicate that CD29, CD44, CD133 and Dlk-1 have potential as suitable markers in cHCC identification, suggesting that these findings will contribute to the establishment of an early diagnostic tool for the identification of hepatocellular maturation processes.

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