Characterization of a Canine Tetranucleotide Microsatellite Marker Located in          the First Intron of the Tumor Necrosis Factor Alpha Gene

Masashi WATANABE; Kazuaki TANAKA; Tatsuya TAKIZAWA; Kazuhito SEGAWA; Sakurako NEO; Ryo TSUCHIYA; Michiko MURATA; Masaru MURAKAMI; Masaharu HISASUE

doi:10.1292/jvms.13-0316

Biochemistry

Characterization of a Canine Tetranucleotide Microsatellite Marker Located in the First Intron of the Tumor Necrosis Factor Alpha Gene

Masashi WATANABE, Kazuaki TANAKA, Tatsuya TAKIZAWA, Kazuhito SEGAWA, Sakurako NEO, Ryo TSUCHIYA, Michiko MURATA, Masaru MURAKAMI, Masaharu HISASUE

Author information

Masashi WATANABE
Laboratory of Veterinary Internal Medicine II, Faculty of Veterinary Medicine, Azabu University, Chuo-ku, Sagamihara, Kanagawa 252–5201, Japan
Kazuaki TANAKA
Laboratory of Animal Biotechnology, Faculty of Veterinary Medicine, Azabu University, Chuo-ku, Sagamihara, Kanagawa 252–5201, Japan
Tatsuya TAKIZAWA
Laboratory of Animal Biotechnology, Faculty of Veterinary Medicine, Azabu University, Chuo-ku, Sagamihara, Kanagawa 252–5201, Japan
Kazuhito SEGAWA
Laboratory of Veterinary Internal Medicine II, Faculty of Veterinary Medicine, Azabu University, Chuo-ku, Sagamihara, Kanagawa 252–5201, Japan
Sakurako NEO
Laboratory of Veterinary Internal Medicine II, Faculty of Veterinary Medicine, Azabu University, Chuo-ku, Sagamihara, Kanagawa 252–5201, Japan
Ryo TSUCHIYA
Laboratory of Veterinary Internal Medicine II, Faculty of Veterinary Medicine, Azabu University, Chuo-ku, Sagamihara, Kanagawa 252–5201, Japan
Michiko MURATA
Laboratory of Molecular Biology, Faculty of Veterinary Medicine, Azabu University, Chuo-ku, Sagamihara, Kanagawa 252–5201, Japan
Masaru MURAKAMI
Laboratory of Molecular Biology, Faculty of Veterinary Medicine, Azabu University, Chuo-ku, Sagamihara, Kanagawa 252–5201, Japan
Masaharu HISASUE
Laboratory of Veterinary Internal Medicine II, Faculty of Veterinary Medicine, Azabu University, Chuo-ku, Sagamihara, Kanagawa 252–5201, Japan

Keywords: canine TNFA gene, tetranucleotide microsatellite

JOURNAL FREE ACCESS

2014 Volume 76 Issue 1 Pages 119-122

DOI https://doi.org/10.1292/jvms.13-0316

Browse “Advance online publication” version

Details

Abstract

A polymorphic tetranucleotide (GAAT)_n microsatellite in the first intron of the canine tumor necrosis factor alpha (TNFA) gene was characterized in this study; 139 dogs were analyzed: 22 Beagles, 26 Chihuahuas, 20 Miniature Dachshunds, 24 Miniature Poodles, 22 Pembroke Welsh Corgis and 25 Shiba Inus. We detected the presence of the 4 alleles (GAAT)₅, (GAAT)₆, (GAAT)₇ and (GAAT)₈, including 9 of the 10 expected genotypes. The expected heterozygosity (He) and the polymorphic information content (PIC) value of this microsatellite locus varied from 0.389 to 0.749 and from 0.333 to 0.682, respectively, among the 6 breeds. The allelic frequency differed greatly among breeds, but this microsatellite marker was highly polymorphic and could be a useful marker for the canine TNFA gene.

Corresponding author

Register with J-STAGE for free!