Implantation of Tetrapod-Shaped Granular Artificial Bones or β-Tricalcium Phosphate Granules in a Canine Large Bone-Defect Model

Sungjin CHOI; I-Li LIU; Kenichi YAMAMOTO; Muneki HONNAMI; Takamasa SAKAI; Shinsuke OHBA; Ryosuke ECHIGO; Shigeki SUZUKI; Ryouhei NISHIMURA; Ung-il CHUNG; Nobuo SASAKI; Manabu MOCHIZUKI

doi:10.1292/jvms.13-0054

Surgery

Implantation of Tetrapod-Shaped Granular Artificial Bones or β-Tricalcium Phosphate Granules in a Canine Large Bone-Defect Model

Sungjin CHOI, I-Li LIU, Kenichi YAMAMOTO, Muneki HONNAMI, Takamasa SAKAI, Shinsuke OHBA, Ryosuke ECHIGO, Shigeki SUZUKI, Ryouhei NISHIMURA, Ung-il CHUNG, Nobuo SASAKI, Manabu MOCHIZUKI

Author information

Sungjin CHOI
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7–3–1 Hongo, Bunkyo-ku, Tokyo 113–0033, Japan
I-Li LIU
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
Kenichi YAMAMOTO
Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7–3–1 Hongo, Bunkyo-ku, Tokyo 113–0033, Japan
Muneki HONNAMI
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
Department of Bioengineering, School of Engineering, The University of Tokyo, 7–3–1 Hongo, Bunkyo-ku, Tokyo 113–0033, Japan
Takamasa SAKAI
Department of Bioengineering, School of Engineering, The University of Tokyo, 7–3–1 Hongo, Bunkyo-ku, Tokyo 113–0033, Japan
Shinsuke OHBA
Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7–3–1 Hongo, Bunkyo-ku, Tokyo 113–0033, Japan
Ryosuke ECHIGO
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
Shigeki SUZUKI
NEXT21 K.K., 3–38–1 Hongo, Bunkyo-ku, Tokyo 113–0033, Japan
Ryouhei NISHIMURA
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
Ung-il CHUNG
Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7–3–1 Hongo, Bunkyo-ku, Tokyo 113–0033, Japan
Department of Bioengineering, School of Engineering, The University of Tokyo, 7–3–1 Hongo, Bunkyo-ku, Tokyo 113–0033, Japan
Nobuo SASAKI
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
Manabu MOCHIZUKI
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan

Keywords: artificial bones, β-TCP granules, canine, critical-size defect, Tetrabone

JOURNAL FREE ACCESS

2014 Volume 76 Issue 2 Pages 229-235

DOI https://doi.org/10.1292/jvms.13-0054

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Abstract

We investigated biodegradability and new bone formation after implantation of tetrapod-shaped granular artificial bone (Tetrabone^®) or β-tricalcium phosphate granules (β-TCP) in experimental critical-size defects in dogs, which were created through medial and lateral femoral condyles. The defect was packed with Tetrabone^® (Tetrabone group) or β-TCP (β-TCP group) or received no implant (control group). Computed tomography (CT) was performed at 0, 4 and 8 weeks after implantation. Micro-CT and histological analysis were conducted to measure the non-osseous tissue rate and the area and distribution of new bone tissue in the defect at 8 weeks after implantation. On CT, β-TCP was gradually resorbed, while Tetrabone^® showed minimal resorption at 8 weeks after implantation. On micro-CT, non-osseous tissue rate of the control group was significantly higher compared with the β-TCP and Tetrabone groups (P<0.01), and that of the β-TCP group was significantly higher compared with the Tetrabone group (P<0.05). On histology, area of new bone tissue of the β-TCP group was significantly greater than those of the Tetrabone and control groups (P<0.05), and new bone distribution of the Tetrabone group was significantly greater than those of the β-TCP and control groups (P<0.05). These results indicate differences in biodegradability and connectivity of intergranule pore structure between study samples. In conclusion, Tetrabone^® may be superior for the repair of large bone defects in dogs.

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