Canine Amino Acid Transport System Xc–: cDNA Sequence,          Distribution and Cystine Transport Activity in Lens Epithelial Cells

Takuya MARUO; Nobuyuki KANEMAKI; Ken ONDA; Reiichiro SATO; Nobuteru ICHIHARA; Hideharu OCHIAI

doi:10.1292/jvms.13-0170

Internal Medicine

Canine Amino Acid Transport System X_c^–: cDNA Sequence, Distribution and Cystine Transport Activity in Lens Epithelial Cells

Takuya MARUO, Nobuyuki KANEMAKI, Ken ONDA, Reiichiro SATO, Nobuteru ICHIHARA, Hideharu OCHIAI

Author information

Keywords: cysteine/glutamate exchanger, glutathione, lens epithelial cells, SLC7A11, system x_c^–

JOURNAL FREE ACCESS

2014 Volume 76 Issue 4 Pages 523-530

DOI https://doi.org/10.1292/jvms.13-0170

Browse “Advance online publication” version

Details

Abstract

The cystine transport activity of a lens epithelial cell line originated from a canine mature cataract was investigated. The distinct cystine transport activity was observed, which was inhibited to 28% by extracellular 1 mM glutamate. The cDNA sequences of canine cysteine/glutamate exchanger (xCT) and 4F2hc were determined. The predicted amino acid sequences were 527 and 533 amino acid polypeptides, respectively. The amino acid sequences of canine xCT and 4F2hc showed high similarities (>80%) to those of humans. The expression of xCT in lens epithelial cell line was confirmed by western blot analysis. RT-PCR analysis revealed high level expression only in the brain, and it was below the detectable level in other tissues.

Corresponding author

Register with J-STAGE for free!