GnRH analogue treatment on LH surge day 0 followed by single transvaginal          artificial insemination with frozen semen on day 5 in bitches

Tadatoshi OHTAKI; Yasuna KOGA; Mamiko ONO; Gen WATANABE; Kazuyoshi TAYA; Shigehisa TSUMAGARI

doi:10.1292/jvms.14-0270

Theriogenology

GnRH analogue treatment on LH surge day 0 followed by single transvaginal artificial insemination with frozen semen on day 5 in bitches

Tadatoshi OHTAKI, Yasuna KOGA, Mamiko ONO, Gen WATANABE, Kazuyoshi TAYA, Shigehisa TSUMAGARI

Author information

Tadatoshi OHTAKI
Laboratory of Theriogenology, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252–0880, Japan
Yasuna KOGA
Laboratory of Theriogenology, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252–0880, Japan
Mamiko ONO
Laboratory of Theriogenology, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252–0880, Japan
Gen WATANABE
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183–8509, Japan
United Graduate School of Veterinary Sciences, Gifu University, Gifu 501–1193, Japan
Kazuyoshi TAYA
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183–8509, Japan
Shadai Corporation, 275 Hayakitagenbu, Yufutsu-gun Abira-cho, Hokkaido 059–1432, Japan
Shigehisa TSUMAGARI
Laboratory of Theriogenology, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252–0880, Japan

Keywords: bitch, Foley catheter, frozen semen, gonadotropin releasing hormone, transvaginal insemination

JOURNAL FREE ACCESS

2015 Volume 77 Issue 1 Pages 123-126

DOI https://doi.org/10.1292/jvms.14-0270

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Abstract

Reproductive parameters were evaluated in 19 and 14 estrous beagles that received 100 µg of gonadotropin-releasing hormone (GnRH) and saline treatment, respectively, on the day of luteinizing hormone (LH) surge (Day 0; estimated by serial progesterone assay) and balloon catheter-aided single transvaginal artificial insemination of frozen semen on Day 5. Although the conception rate and litter size were similar between the GnRH and saline groups, the concentration of LH peak was significantly higher in GnRH-treated bitches (P<0.01). In addition, the actual LH surge did not occur on the estimated Day 0 in one saline-treated bitch. In clinical practice that daily progesterone assay is difficult, administration of GnRH on estimated Day 0 would be recommended to induce or enhance the LH surge for timely and successful insemination.

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