Expression and significance of CHIP in canine mammary gland          tumors

Huanan WANG; Xu YANG; Yipeng JIN; Shimin PEI; Di ZHANG; Wen MA; Jian HUANG; Hengbin QIU; Xinke ZHANG; Qiuyue JIANG; Weidong SUN; Hong ZHANG; Degui LIN

doi:10.1292/jvms.14-0484

Pathology

Expression and significance of CHIP in canine mammary gland tumors

Huanan WANG, Xu YANG, Yipeng JIN, Shimin PEI, Di ZHANG, Wen MA, Jian HUANG, Hengbin QIU, Xinke ZHANG, Qiuyue JIANG, Weidong SUN, Hong ZHANG, Degui LIN

著者情報

Huanan WANG
The Clinical Department, College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R. China
Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310012, P.R. China
Xu YANG
The Clinical Department, College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R. China
Yipeng JIN
The Clinical Department, College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R. China
Shimin PEI
The Clinical Department, College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R. China
Di ZHANG
The Clinical Department, College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R. China
Wen MA
The Clinical Department, College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R. China
Jian HUANG
The Clinical Department, College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R. China
Hengbin QIU
The Clinical Department, College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R. China
Xinke ZHANG
The Clinical Department, College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R. China
Qiuyue JIANG
The Clinical Department, College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R. China
Weidong SUN
The Clinical Department, College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R. China
Hong ZHANG
The Clinical Department, College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R. China
Degui LIN
The Clinical Department, College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R. China

キーワード: canine mammary gland tumor, CHIP, immunohistochemistry, prognosis, RT-PCR

ジャーナルフリー

2015 年 77 巻 11 号 p. 1465-1471

DOI https://doi.org/10.1292/jvms.14-0484

Browse “Advance online publication” version

詳細

抄録

CHIP (Carboxy terminus of Hsc70 Interacting Protein) is an E3 ubiquitin ligase that can induce ubiquitination and degradation of several oncogenic proteins. The expression of CHIP is frequently lower in human breast cancer than in normal breast tissue. However, the expression and role of CHIP in the canine mammary gland tumor (CMGT) remain unclear. We investigated the potential correlation between CHIP expression and mammary gland tumor prognosis in female dogs. CHIP expression was measured in 54 dogs by immunohistochemistry and real-time RT-PCR. CHIP protein expression was significantly correlated with the histopathological diagnosis, outcome of disease and tumor classification. The transcriptional level of CHIP was significantly higher in normal tissues (P=0.001) and benign tumors (P=0.009) than it in malignant tumors. CHIP protein expression was significantly correlated with the transcriptional level of CHIP (P=0.0102). The log-rank test survival curves indicated that patients with low expression of CHIP had shorter overall periods of survival than those with higher CHIP protein expression (P=0.050). Our data suggest that CHIP may play an important role in the formation and development of CMGTs and serve as a valuable prognostic marker and potential target for genetic therapy.

責任著者(Corresponding author)

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