Relationship between three novel SNPs of BRCA1 and canine mammary          tumors

Weidong SUN; Xu YANG; Hengbin QIU; Di ZHANG; Huanan WANG; Jian HUANG; Degui LIN

doi:10.1292/jvms.15-0044

Biochemistry

Relationship between three novel SNPs of BRCA1 and canine mammary tumors

Weidong SUN, Xu YANG, Hengbin QIU, Di ZHANG, Huanan WANG, Jian HUANG, Degui LIN

Author information

Weidong SUN
College of Veterinary Medicine, China Agricultural University, 2 Yuanmingyuan Xilu, Haidian District, Beijing 100193, P. R. China
Xu YANG
College of Veterinary Medicine, China Agricultural University, 2 Yuanmingyuan Xilu, Haidian District, Beijing 100193, P. R. China
Hengbin QIU
College of Veterinary Medicine, China Agricultural University, 2 Yuanmingyuan Xilu, Haidian District, Beijing 100193, P. R. China
Di ZHANG
College of Veterinary Medicine, China Agricultural University, 2 Yuanmingyuan Xilu, Haidian District, Beijing 100193, P. R. China
Huanan WANG
College of Veterinary Medicine, China Agricultural University, 2 Yuanmingyuan Xilu, Haidian District, Beijing 100193, P. R. China
Jian HUANG
College of Veterinary Medicine, China Agricultural University, 2 Yuanmingyuan Xilu, Haidian District, Beijing 100193, P. R. China
Degui LIN
College of Veterinary Medicine, China Agricultural University, 2 Yuanmingyuan Xilu, Haidian District, Beijing 100193, P. R. China

Keywords: breast cancer susceptibility gene 1 (BRCA1), canine mammary tumors (CMTs), rapid amplification of cDNA ends (RACE), single-nucleotide polymorphisms (SNPs), untranslated region (UTR)

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Supplementary material

2015 Volume 77 Issue 11 Pages 1541-1543

DOI https://doi.org/10.1292/jvms.15-0044

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Abstract

The BRCA1 gene plays an important role in the development of human breast cancer, and recent research indicated that genetic variations of BRCA1 are also related to canine mammary tumors (CMTs). Here, using rapid amplification of cDNA ends (RACE), we cloned the 5′- and 3′-UTRs of BRCA1. By direct sequencing of the flanking sequences of the 5′- and 3′-UTRs of BRCA1, three previously unreported single-nucleotide polymorphisms (SNPs) were identified, two (−1228T >C, −1173C >T) in the putative promoter regions and one non-synonymous SNP (63449G >A) in exon 23. Compared with 16 normal samples, the sequences from 34 CMTs suggested that SNP (−1173C >T) was associated with the development of CMTs (odds ratio (OR)=2.57, 95% confidence interval (CI): 1.07–6.15).

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