Application of crude and recombinant ELISAs and immunochromatographic test for serodiagnosis of animal trypanosomosis in the Umkhanyakude district of KwaZulu-Natal province, South Africa

Thu-Thuy NGUYEN; Mono Sophie MOTSIRI; Moeti Oriel TAIOE; Moses Sibusiso MTSHALI; Yasuyuki GOTO; Shin-Ichiro KAWAZU; Oriel Matlhahane Molifi THEKISOE; Noboru INOUE

doi:10.1292/jvms.14-0330

Parasitology

Application of crude and recombinant ELISAs and immunochromatographic test for serodiagnosis of animal trypanosomosis in the Umkhanyakude district of KwaZulu-Natal province, South Africa

Thu-Thuy NGUYEN, Mono Sophie MOTSIRI, Moeti Oriel TAIOE, Moses Sibusiso MTSHALI, Yasuyuki GOTO, Shin-Ichiro KAWAZU, Oriel Matlhahane Molifi THEKISOE, Noboru INOUE

著者情報

Thu-Thuy NGUYEN
OIE Reference Laboratory for Surra, OIE Collaborating Centre, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
Mono Sophie MOTSIRI
Parasitology Research Program, Department of Zoology and Entomology, University of the Free State–Qwaqwa Campus, Private Bag, X13, Phuthaditjhaba 9866, South Africa
Moeti Oriel TAIOE
Parasitology Research Program, Department of Zoology and Entomology, University of the Free State–Qwaqwa Campus, Private Bag, X13, Phuthaditjhaba 9866, South Africa
Moses Sibusiso MTSHALI
Parasitology Research Program, Department of Zoology and Entomology, University of the Free State–Qwaqwa Campus, Private Bag, X13, Phuthaditjhaba 9866, South Africa
Veterinary Parasitology Unit, Research and Scientific Services Department, National Zoological Gardens of South Africa, Pretoria 0001, South Africa
Yasuyuki GOTO
Laboratory of Molecular Immunology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
Shin-Ichiro KAWAZU
OIE Reference Laboratory for Surra, OIE Collaborating Centre, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
Oriel Matlhahane Molifi THEKISOE
Parasitology Research Program, Department of Zoology and Entomology, University of the Free State–Qwaqwa Campus, Private Bag, X13, Phuthaditjhaba 9866, South Africa
Noboru INOUE
OIE Reference Laboratory for Surra, OIE Collaborating Centre, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan

キーワード: animal African trypanosomosis, ELISA, immunochromatographic test, serodiagnosis, South Africa

ジャーナルフリー

2015 年 77 巻 2 号 p. 217-220

DOI https://doi.org/10.1292/jvms.14-0330

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詳細

抄録

A total of 231 serum samples were collected from sheep (n=9), goats (n=99) and cattle (n=123) in northeastern KwaZulu-Natal, South Africa. Trypanosome infection was detected using Trypanosoma brucei brucei crude antigen (TbbCA) and T. congolense crude antigen (TcoCA) ELISA assays. Recombinant antigen (T. evansi GM6 which consisted of 4 repeat domains, TeGM6-4r) ELISA and immunochromatographic test (ICT) were also used. Crude antigen ELISA, TeGM6-4r-ELISA and ICT detected 27.3%, 29% and 19.9% of trypanosome seropositive samples, respectively. Trypanosome infection prevalence in cattle and goats was 35.8–46.3% and 0–9.1%, respectively. Out of 9 sheep serum samples, 2–4 sera (22.2–44.4%) were positive. The detection performance of crude and recombinant antigen ELISAs was relatively similar (K=0.6–0.7); both are recommended for reference diagnosis and large scale epidemiological surveys. There is potential application for ICT in on-site diagnosis, but its sensitivity should be improved.

責任著者(Corresponding author)

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