Enzyme-linked immunosorbent assays using recombinant TgSAG2 and NcSAG1 to          detect Toxoplasma gondii and Neospora caninum-specific          antibodies in domestic animals in Turkey

Mo ZHOU; Shinuo CAO; Ferda SEVINC; Mutlu SEVINC; Onur CEYLAN; Mingming LIU; Guanbo WANG; Paul Franck Adjou MOUMOUNI; Charoonluk JIRAPATTHARASATE; Hiroshi SUZUKI; Yoshifumi NISHIKAWA; Xuenan XUAN

doi:10.1292/jvms.16-0234

Parasitology

Enzyme-linked immunosorbent assays using recombinant TgSAG2 and NcSAG1 to detect Toxoplasma gondii and Neospora caninum-specific antibodies in domestic animals in Turkey

Mo ZHOU, Shinuo CAO, Ferda SEVINC, Mutlu SEVINC, Onur CEYLAN, Mingming LIU, Guanbo WANG, Paul Franck Adjou MOUMOUNI, Charoonluk JIRAPATTHARASATE, Hiroshi SUZUKI, Yoshifumi NISHIKAWA, Xuenan XUAN

Author information

Mo ZHOU
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
Shinuo CAO
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
Ferda SEVINC
Selcuk University, Faculty of Veterinary Medicine, Konya, Turkey
Mutlu SEVINC
Selcuk University, Faculty of Veterinary Medicine, Konya, Turkey
Onur CEYLAN
Selcuk University, Faculty of Veterinary Medicine, Konya, Turkey
Mingming LIU
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
Guanbo WANG
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
Paul Franck Adjou MOUMOUNI
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
Charoonluk JIRAPATTHARASATE
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
Hiroshi SUZUKI
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
Yoshifumi NISHIKAWA
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
Xuenan XUAN
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan

Keywords: ELISA, Neospora caninum, Toxoplasma gondii, Turkey

JOURNAL FREE ACCESS

2016 Volume 78 Issue 12 Pages 1877-1881

DOI https://doi.org/10.1292/jvms.16-0234

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Abstract

Considering the scarce information on occurrences of Toxoplasma gondii and Neospora caninum in domestic animals from Turkey, the aim of this study was to investigate the seroprevalence of these parasite infections in cattle, horses, sheep, goats and dogs in Turkey. The specific antibodies against T. gondii and N. caninum were detected by iELISAs based on the recombinant TgSAG2 or NcSAG1 in a total of 2,039 serum samples from eleven provinces. The seroprevalence of T. gondii infections was 46.3%, 4.0%, 20.0%, 12.9% and 19.8%, that of N. caninum infections was 0.3%, 7.4%, 2.1%, 3.2% and 16.6% in the horses, cattle, sheep, goats and dogs, respectively. These results indicated that T. gondii and N. caninum infections are prevalent in Turkish domestic animals.

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