Detection of circulating tumor cells using GeneScan analysis for antigen          receptor gene rearrangements in canine lymphoma patients

Saaya HIYOSHI-KANEMOTO; Yuko GOTO-KOSHINO; Kenjiro FUKUSHIMA; Masashi TAKAHASHI; Hideyuki KANEMOTO; Kazuyuki UCHIDA; Yasuhito FUJINO; Koichi OHNO; Hajime TSUJIMOTO

doi:10.1292/jvms.15-0433

Internal Medicine

Detection of circulating tumor cells using GeneScan analysis for antigen receptor gene rearrangements in canine lymphoma patients

Saaya HIYOSHI-KANEMOTO, Yuko GOTO-KOSHINO, Kenjiro FUKUSHIMA, Masashi TAKAHASHI, Hideyuki KANEMOTO, Kazuyuki UCHIDA, Yasuhito FUJINO, Koichi OHNO, Hajime TSUJIMOTO

Author information

Saaya HIYOSHI-KANEMOTO
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
Yuko GOTO-KOSHINO
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
Kenjiro FUKUSHIMA
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
Masashi TAKAHASHI
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
Hideyuki KANEMOTO
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
Kazuyuki UCHIDA
Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
Yasuhito FUJINO
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
Koichi OHNO
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
Hajime TSUJIMOTO
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan

Keywords: antigen receptor gene rearrangements, canine, circulating tumor cells, GeneScan, lymphoma

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Supplementary material

2016 Volume 78 Issue 5 Pages 877-881

DOI https://doi.org/10.1292/jvms.15-0433

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Abstract

The presence of circulating tumor cells (CTCs) serves as a prognostic marker and indicator of disease relapse, as well as a means of evaluating treatment efficacy in human and canine lymphoma patients. As an extension of our previous study for the construction of clinically useful GeneScan system, we utilized the GeneScan system for detecting CTCs in canine lymphoma patients. Samples from the primary lesion and peripheral blood mononuclear cells (PBMCs) were obtained from 32 dogs with lymphoma at initial diagnosis. All samples were subjected to polymerase chain reaction (PCR) for antigen receptor gene rearrangements (PARR) followed by GeneScan analysis. Common clonal rearrangements with identical amplified fragments were detected in both the primary lesion and PBMCs in 19 of the 32 dogs (59.4%). However, the detection rate of CTCs varied among the anatomical classification of lymphoma studied. GeneScan analysis following PARR would facilitate studies on determining the clinical significance of CTCs in canine lymphoma patients.

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