Migratory ability of gonadal germ cells (GGCs) isolated from Ciconia boyciana and Geronticus eremita embryos into the gonad of developing chicken embryos

Yuki NAKAJIMA; Haruka FUKUDA; Manabu ONUMA; Koichi MURATA; Miya UEDA; Emi SUNAGA; Toshirou SHIRAISHI; Atsushi TAJIMA

doi:10.1292/jvms.15-0664

Wildlife Science

Migratory ability of gonadal germ cells (GGCs) isolated from Ciconia boyciana and Geronticus eremita embryos into the gonad of developing chicken embryos

Yuki NAKAJIMA, Haruka FUKUDA, Manabu ONUMA, Koichi MURATA, Miya UEDA, Emi SUNAGA, Toshirou SHIRAISHI, Atsushi TAJIMA

Author information

Yuki NAKAJIMA
Faculty of Life and Environmental Sciences, University of Tsukuba, Ten-noh Dai 1–1–1, Tsukuba, Ibaraki 305–8572, Japan
Fellow of the Japanese Society for the Promotion of Science, Japan
Current address: Advanced Biological Information Research Division, INAMORI Frontier Research Center, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819–0395, Japan
Haruka FUKUDA
Graduate School of Life and Environmental Sciences, University of Tsukuba, Ten-noh Dai 1–1–1, Tsukuba, Ibaraki 305–8572, Japan
Manabu ONUMA
Center for Environmental Biology and Ecosystem Studies, National Institute for Environmental Studies, 16–2 Onogawa, Tsukuba, Ibaraki 305–8506, Japan
Koichi MURATA
Zoorasia Yokohama Zoological Gardens, Kamishiranecho 1175–1, Yokohama Asahi-ku, Kanagawa 241–0001, Japan
Preservation and Research Center/The City of Yokohama, Kawaishukucho 155–1, Yokohama Asahi-ku, Kanagawa 241–0804, Japan
College of Bioresource Sciences, Nihon University, Kameino 1866, Fujisawa, Kanagawa, 252–0880, Japan
Miya UEDA
Zoorasia Yokohama Zoological Gardens, Kamishiranecho 1175–1, Yokohama Asahi-ku, Kanagawa 241–0001, Japan
Emi SUNAGA
Preservation and Research Center/The City of Yokohama, Kawaishukucho 155–1, Yokohama Asahi-ku, Kanagawa 241–0804, Japan
Toshirou SHIRAISHI
Preservation and Research Center/The City of Yokohama, Kawaishukucho 155–1, Yokohama Asahi-ku, Kanagawa 241–0804, Japan
Atsushi TAJIMA
Faculty of Life and Environmental Sciences, University of Tsukuba, Ten-noh Dai 1–1–1, Tsukuba, Ibaraki 305–8572, Japan

Keywords: chicken, Ciconia boyciana, Geronticus eremita, gonadal germ cells (GGCs), migratory ability

JOURNAL FREE ACCESS

2016 Volume 78 Issue 6 Pages 1055-1058

DOI https://doi.org/10.1292/jvms.15-0664

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Abstract

We conducted experiments to evaluate the ability of gonadal germ cells (GGCs), isolated from the embryonic gonads of Ciconia boyciana or Geronticus eremita, to migrate into the gonads of developing chicken embryos. Fluorescently labeled GGCs, isolated by the PBS (−) method, were transferred into the dorsal aorta of 2-day-old chicken embryos. Five days after transfer, fluorescent GGCs were detected in the gonads of recipient embryos. Our results indicate that GGCs from Ciconia boyciana and Geronticus eremita are capable of migrating into the gonads of developing chicken embryos.

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