Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
Wildlife Science
Development and evaluation of an interferon-γ release assay in Asian elephants (Elephas maximus)
Sarad PAUDELMarvin A. VILLANUEVASusan K. MIKOTAChie NAKAJIMAKamal P. GAIRHESuraj SUBEDINabin RAYAMAJHIMariko SASHIKAMichito SHIMOZURUTakashi MATSUBAYasuhiko SUZUKIToshio TSUBOTA
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Volume 78 (2016) Issue 7 Pages 1117-1121

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Abstract

We developed an interferon-γ release assay (IGRA) specific for Asian elephants (Elephas maximus). Whole blood collected from forty captive Asian elephants was stimulated with three different mitogens i.e., phytohemagglutinin (PHA), pokweed mitogen (PWM) and phorbol myristate aceteate/ionomycin (PMA/I). A sandwich ELISA that was able to recognize the recombinant elephant interferon-γ (rEIFN-γ) as well as native interferon-γ from the Asian elephants was performed using anti-elephant IFN-γ rabbit polyclonal antibodies as capture antibodies and biotinylated anti-elephant IFN-γ rabbit polyclonal antibodies as detection antibodies. PMA/I was the best mitogen to use as a positive control for an Asian elephant IGRA. The development of an Asian elephant-specific IGRA that detects native IFN-γ in elephant whole blood provides promising results for its application as a potential diagnostic tool for diseases, such as tuberculosis (TB) in Asian elephants.

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