Development and evaluation of an interferon-γ release assay in Asian          elephants (Elephas maximus)

Sarad PAUDEL; Marvin A. VILLANUEVA; Susan K. MIKOTA; Chie NAKAJIMA; Kamal P. GAIRHE; Suraj SUBEDI; Nabin RAYAMAJHI; Mariko SASHIKA; Michito SHIMOZURU; Takashi MATSUBA; Yasuhiko SUZUKI; Toshio TSUBOTA

doi:10.1292/jvms.15-0701

Wildlife Science

Development and evaluation of an interferon-γ release assay in Asian elephants (Elephas maximus)

Sarad PAUDEL, Marvin A. VILLANUEVA, Susan K. MIKOTA, Chie NAKAJIMA, Kamal P. GAIRHE, Suraj SUBEDI, Nabin RAYAMAJHI, Mariko SASHIKA, Michito SHIMOZURU, Takashi MATSUBA, Yasuhiko SUZUKI, Toshio TSUBOTA

著者情報

Sarad PAUDEL
Laboratory of Wildlife Biology and Medicine, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo, Hokkaido 060–0818, Japan
Marvin A. VILLANUEVA
Division of Bioresources, Hokkaido University Research Center for Zoonosis Control, Kita 20, Nishi 10, Kita-ku, Sapporo, Hokkaido 001–0020, Japan
Susan K. MIKOTA
Elephant Care International, 166 Limo View Lane, Hohenwald, TN 38462, U.S.A.
Chie NAKAJIMA
Division of Bioresources, Hokkaido University Research Center for Zoonosis Control, Kita 20, Nishi 10, Kita-ku, Sapporo, Hokkaido 001–0020, Japan
Hokkaido University The Global station for Zoonosis Control, Sapporo, Japan
Kamal P. GAIRHE
Department of National Parks and Wildlife Conservation, Babarmahal, Kathmandu, Nepal
Suraj SUBEDI
National Trust for Nature Conservation, Lalitpur, Nepal
Nabin RAYAMAJHI
Patan Academy of Health Sciences, Patan, Nepal
Mariko SASHIKA
Laboratory of Wildlife Biology and Medicine, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo, Hokkaido 060–0818, Japan
Michito SHIMOZURU
Laboratory of Wildlife Biology and Medicine, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo, Hokkaido 060–0818, Japan
Takashi MATSUBA
Division of Bacteriology, Department of Microbiology and Immunology, Faculty of Medicine, Tottori University, Yonago, Tottori, Japan
Yasuhiko SUZUKI
Division of Bioresources, Hokkaido University Research Center for Zoonosis Control, Kita 20, Nishi 10, Kita-ku, Sapporo, Hokkaido 001–0020, Japan
Hokkaido University The Global station for Zoonosis Control, Sapporo, Japan
Toshio TSUBOTA
Laboratory of Wildlife Biology and Medicine, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo, Hokkaido 060–0818, Japan

キーワード: Asian elephant, interferon-γ, mitogen, sandwich ELISA

ジャーナルフリー

2016 年 78 巻 7 号 p. 1117-1121

DOI https://doi.org/10.1292/jvms.15-0701

Browse “Advance online publication” version

詳細

抄録

We developed an interferon-γ release assay (IGRA) specific for Asian elephants (Elephas maximus). Whole blood collected from forty captive Asian elephants was stimulated with three different mitogens i.e., phytohemagglutinin (PHA), pokweed mitogen (PWM) and phorbol myristate aceteate/ionomycin (PMA/I). A sandwich ELISA that was able to recognize the recombinant elephant interferon-γ (rEIFN-γ) as well as native interferon-γ from the Asian elephants was performed using anti-elephant IFN-γ rabbit polyclonal antibodies as capture antibodies and biotinylated anti-elephant IFN-γ rabbit polyclonal antibodies as detection antibodies. PMA/I was the best mitogen to use as a positive control for an Asian elephant IGRA. The development of an Asian elephant-specific IGRA that detects native IFN-γ in elephant whole blood provides promising results for its application as a potential diagnostic tool for diseases, such as tuberculosis (TB) in Asian elephants.

責任著者(Corresponding author)

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