Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
Internal Medicine
Analysis of lipoprotein profiles of healthy cats by gel-permeation high-performance liquid chromatography
Hisashi MIZUTANIToshinori SAKOHiroko OKUDANobuaki ARAIKoji KURIYAMAAkihiro MORIItaru YOSHIMURAHidekazu KOYAMA
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2016 Volume 78 Issue 8 Pages 1277-1281

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Abstract

Density gradient ultracentrifugation (DGUC) and gel electrophoresis are conventionally used to obtain lipoprotein profiles of animals. We recently applied high-performance liquid chromatography with a gel permeation column (GP-HPLC) and an on-line dual enzymatic system to dogs for lipoprotein profile analysis. We compared the GP-HPLC with DGUC as a method to obtain a feline lipoprotein profile. The lipoprotein profiles showed large and small peaks, which corresponded to high-density lipoprotein (HDL) and low-density lipoprotein (LDL), respectively, whereas very low-density lipoprotein (VLDL) and chylomicron (CM) were only marginally detected. This profile was very similar to that of dogs reported previously. Healthy cats also had a small amount of cholesterol-rich particles distinct from the normal LDL or HDL profile. There was no difference in lipoprotein profiles between the sexes, but males had a significantly larger LDL particle size (P=0.015). This study shows the feasibility of GP-HPLC for obtaining accurate lipoprotein profiles with small sample volumes and provides valuable reference data for healthy cats that should facilitate diagnoses.

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