Validation of an HPLC assay for determination of Telazol in pregnant pigs:          application to placental transfer study

Wei LI; Gangshi LI; Zhijun ZHONG; Bingbing XIE; Ziyao ZHOU; Wuyang GU; Xianpeng SHI; Tianliang TANG; Shengquan AI; Hualin FU; Mengjiao LIU; Mengxi LIU; De WU; Yanchun HU; Guangneng PENG

doi:10.1292/jvms.16-0300

Laboratory Animal Science

Validation of an HPLC assay for determination of Telazol in pregnant pigs: application to placental transfer study

Wei LI, Gangshi LI, Zhijun ZHONG, Bingbing XIE, Ziyao ZHOU, Wuyang GU, Xianpeng SHI, Tianliang TANG, Shengquan AI, Hualin FU, Mengjiao LIU, Mengxi LIU, De WU, Yanchun HU, Guangneng PENG

Author information

Wei LI
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, P. R. China
Gangshi LI
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, P. R. China
Zhijun ZHONG
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, P. R. China
Bingbing XIE
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, P. R. China
Ziyao ZHOU
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, P. R. China
Wuyang GU
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, P. R. China
Xianpeng SHI
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, P. R. China
Tianliang TANG
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, P. R. China
Shengquan AI
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, P. R. China
Hualin FU
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, P. R. China
Mengjiao LIU
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, P. R. China
Mengxi LIU
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, P. R. China
De WU
Institute of Animal Nutrition, Sichuan Agricultural University, Ya’an 625014, P. R. China
Yanchun HU
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, P. R. China
Guangneng PENG
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, P. R. China

Keywords: HPLC, placental barrier, pregnant pigs, tiletamine, zolazepam

JOURNAL FREE ACCESS

2017 Volume 79 Issue 4 Pages 801-806

DOI https://doi.org/10.1292/jvms.16-0300

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Abstract

Anesthetics utilized for the immobilization of pregnant mammals are prone to crossing the placental barrier and cause adverse effects to the fetuses. In this study, we develop a facile method employing high performance liquid chromatography (HPLC) for the study of Telazol crossing the placental barrier of pregnant pigs. The method mainly relies on the efficient extraction strategy that includes the mobile phase composed of 10 mM ammonium acetate aqueous solution-acetonitrile (1:4, v/v). When the injected dose of Telazol is 10 mg/kg (5 mg/kg of each constituent drug, zolazepem and tiletamine), zolazepam can cross the placental barrier as it is detected in both uterus and umbilical cord with approximately the same content. Conversely, tiletamine is detected in neither uterus nor umbilical cord, indicating the absence of placental transfer of tiletamine. The different absorption rates of the two dosage-equal compounds by pigs are found to be the main cause of their different abilities to cross the placental barrier.

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