Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250
Biochemistry
An efficient method for saliva collection from mature pigs to determine their enzymatic and electrolytic profiles
Nadeem AKHTARMohsen JAFARIKIABrian P. SULLIVANJulang LI
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2018 Volume 80 Issue 1 Pages 147-151

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Abstract

In this study, a novel ‘rope-assisted swab method’ for the collection of saliva samples from 45 adult pigs was established and validated. This method was efficient for harvesting 2 milliliters or more of saliva from each of the pigs for subsequent analyses within two min. The amount of α-amylase and lipase in the collected saliva samples was between 38−6,655 and 2−52 U/l, respectively. For HCO3 and electrolytes, the range was between 9−40, 15−76, 4.3−48.5 and 7−65 mM for HCO3, Na+, K+ and Cl, respectively. No significant differences in the enzymatic and electrolytic profiles were observed among sows with a high average litter size (SHA), sows with a low average litter size (SLA) and non-pregnant gilts (NPG) in this study. Our work reveals the efficiency of this collection method for mature pigs, and enzymatic and electrolytic profiling of saliva, which may be a useful reference for multiple diagnostic applications.

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この記事はクリエイティブ・コモンズ [表示 - 非営利 - 改変禁止 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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