Follicle sinus complexes (FSCs) in muzzle skin as postmortem diagnostic          material of rabid dogs

Nozomi SHIWA; Chikage NAKAJIMA; Kazunori KIMITSUKI; Daria Llenaresas MANALO; Akira NOGUCHI; Satoshi INOUE; Chun-Ho PARK

doi:10.1292/jvms.18-0519

Pathology

Follicle sinus complexes (FSCs) in muzzle skin as postmortem diagnostic material of rabid dogs

Nozomi SHIWA, Chikage NAKAJIMA, Kazunori KIMITSUKI, Daria Llenaresas MANALO, Akira NOGUCHI, Satoshi INOUE, Chun-Ho PARK

Author information

Nozomi SHIWA
Department of Veterinary Pathology, School of Veterinary Medicine, Kitasato University, 23-35-1, Higashi, Towada, Aomori 034-8628, Japan
Chikage NAKAJIMA
Department of Veterinary Pathology, School of Veterinary Medicine, Kitasato University, 23-35-1, Higashi, Towada, Aomori 034-8628, Japan
Kazunori KIMITSUKI
Department of Veterinary Pathology, School of Veterinary Medicine, Kitasato University, 23-35-1, Higashi, Towada, Aomori 034-8628, Japan
Daria Llenaresas MANALO
Veterinary Research Department, Research Institute for Tropical Medicine, Department of Health, 9002 Research Drive, Filinvest Corporate City, Alabang, Muntinlupa City 1781, Philippines
Akira NOGUCHI
Department of Veterinary Science, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan
Satoshi INOUE
Department of Veterinary Science, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan
Chun-Ho PARK
Department of Veterinary Pathology, School of Veterinary Medicine, Kitasato University, 23-35-1, Higashi, Towada, Aomori 034-8628, Japan

Keywords: follicle-sinus complex (FSC), Merkel cell, post-mortem diagnosis, rabid dog

JOURNAL FREE ACCESS

2018 Volume 80 Issue 12 Pages 1818-1821

DOI https://doi.org/10.1292/jvms.18-0519

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Abstract

Recently, we reported that follicle-sinus complexes (FSCs) in the muzzle skin are useful for postmortem diagnosis of rabid dogs. Here, we compared the sensitivity and specificity of detecting the viral antigen in the brain and FSCs of 226 suspected rabid dogs, and assessed whether the FSC harbored the virus genome and particles. The viral antigen was detected in 211 of 226 samples with 100% sensitivity and specificity. Viral RNA and particles were observed in the cytoplasm of Merkel cells (MCs). These results suggest that MCs are targets of virus infection and FSCs are useful material for diagnosing rabies.

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