Sound exposure-induced cytokine gene transcript profile changes in captive          bottlenose dolphin (Tursiops truncatus) blood identified by a probe-based          qRT-PCR

I-Hua CHEN; Lien-Siang CHOU; Shih-Jen CHOU; Jiann-Hsiung WANG; Jeffrey STOTT; Myra BLANCHARD; I-Fan JEN; Wei-Cheng YANG

doi:10.1292/jvms.17-0548

Wildlife Science

Sound exposure-induced cytokine gene transcript profile changes in captive bottlenose dolphin (Tursiops truncatus) blood identified by a probe-based qRT-PCR

I-Hua CHEN, Lien-Siang CHOU, Shih-Jen CHOU, Jiann-Hsiung WANG, Jeffrey STOTT, Myra BLANCHARD, I-Fan JEN, Wei-Cheng YANG

Author information

I-Hua CHEN
Department of Veterinary Medicine, National Chiayi University, No. 580, Xinmin Rd., Chiayi, Taiwan (R.O.C.)
Lien-Siang CHOU
Institute of Ecology and Evolutionary Biology, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei, Taiwan (R.O.C.)
Shih-Jen CHOU
Department of Veterinary Medicine, National Chiayi University, No. 580, Xinmin Rd., Chiayi, Taiwan (R.O.C.)
Jiann-Hsiung WANG
Department of Veterinary Medicine, National Chiayi University, No. 580, Xinmin Rd., Chiayi, Taiwan (R.O.C.)
Jeffrey STOTT
School of Veterinary Medicine, University of California, Davis, One Shields Avenue, Davis, CA 95616, U.S.A.
Myra BLANCHARD
School of Veterinary Medicine, University of California, Davis, One Shields Avenue, Davis, CA 95616, U.S.A.
I-Fan JEN
Farglory Ocean Park, No.189, Yenliao Village, Shoufong, Hualien, Taiwan (R.O.C.)
Wei-Cheng YANG
Department of Veterinary Medicine, National Chiayi University, No. 580, Xinmin Rd., Chiayi, Taiwan (R.O.C.)

Keywords: cytokine, dolphin, immune response, qRT-PCR, sound

JOURNAL FREE ACCESS

2018 Volume 80 Issue 4 Pages 601-605

DOI https://doi.org/10.1292/jvms.17-0548

Browse “Advance online publication” version

Details

Abstract

Cetacean health may be potentially affected by anthropogenic sound. We have initiated investigations on the effect of low-frequency underwater sound on immunological gene transcript profiles of captive bottlenose dolphins (Tursiops truncatus) using a probe-based quantitative gene expression assay. Six immunologic genes (IL-2Rα, -4, -10, -12, TNFα and IFNγ) were selected for analysis using two validated housekeeping genes (PGK1 and HPRT1) as reference genes. Twenty-four blood samples from six clinically healthy individuals and six blood samples from individuals after sound exposures were available. The gene transcript profile of sound-exposed dolphins was consistent with a stress-induced T_H2 shift profile as compared to controls. This study may lead to better understanding of the effects of anthropogenic sound on immune responses of cetaceans.

Corresponding author