Establishment of a Madin–Darby bovine kidney cell line expressing anchorless          bovine prion protein

Hyo-Jin KIM; In-Soon ROH; Hoo-Chang PARK; Su Bi AHN; Tae-Young SUH; Kyung-Je PARK; Hae-Eun KANG; Hyun-Joo SOHN

doi:10.1292/jvms.17-0521

Virology

Establishment of a Madin–Darby bovine kidney cell line expressing anchorless bovine prion protein

Hyo-Jin KIM, In-Soon ROH, Hoo-Chang PARK, Su Bi AHN, Tae-Young SUH, Kyung-Je PARK, Hae-Eun KANG, Hyun-Joo SOHN

著者情報

Hyo-Jin KIM
OIE Reference Laboratory for CWD, Foreign Animal Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbukdo 39660, Korea
In-Soon ROH
OIE Reference Laboratory for CWD, Foreign Animal Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbukdo 39660, Korea
Hoo-Chang PARK
OIE Reference Laboratory for CWD, Foreign Animal Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbukdo 39660, Korea
Su Bi AHN
OIE Reference Laboratory for CWD, Foreign Animal Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbukdo 39660, Korea
Tae-Young SUH
OIE Reference Laboratory for CWD, Foreign Animal Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbukdo 39660, Korea
Kyung-Je PARK
OIE Reference Laboratory for CWD, Foreign Animal Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbukdo 39660, Korea
Hae-Eun KANG
OIE Reference Laboratory for CWD, Foreign Animal Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbukdo 39660, Korea
Hyun-Joo SOHN
OIE Reference Laboratory for CWD, Foreign Animal Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbukdo 39660, Korea

キーワード: bovine, GPI anchorless, lentivirus, PrP

ジャーナルフリー

2018 年 80 巻 6 号 p. 909-912

DOI https://doi.org/10.1292/jvms.17-0521

Browse “Advance online publication” version

詳細

抄録

Enzyme-linked immunosorbent assay (ELISA) performed using extensively purified bacterially expressed bovine prion protein (PrP) shows decreased cross-reactivity. We generated a transduced Madin–Darby bovine kidney (MDBK) cell line continuously expressing glycosylphosphatidylinositol (GPI)-anchorless bovine PrP (designated as MDBK ∆GPI protein) by using a lentiviral expression system. The present study also described the method for purifying bovine PrP through sequential culturing without the need for complex purification protocol. Our results showed that the purified bovine PrP could be used as an immunogen for developing anti-PrP monoclonal antibodies. Together, our results suggest that the new GPI-anchorless bovine PrP and its purification method can be used for performing basic studies for employing a cell-based approach.

責任著者(Corresponding author)

J-STAGEへの登録はこちら（無料）