Investigation of genetic diversity and epidemiological characteristics of            Pasteurella multocida isolates from poultry in southwest China by          population structure, multi-locus sequence typing and virulence-associated gene profile          analysis

Zhangcheng LI; Fangjun CHENG; Shimei LAN; Jianhua GUO; Wei LIU; Xiaoyan LI; Zeli LUO; Manli ZHANG; Juan WU; Yang SHI

doi:10.1292/jvms.18-0049

Bacteriology

Investigation of genetic diversity and epidemiological characteristics of Pasteurella multocida isolates from poultry in southwest China by population structure, multi-locus sequence typing and virulence-associated gene profile analysis

Zhangcheng LI, Fangjun CHENG, Shimei LAN, Jianhua GUO, Wei LIU, Xiaoyan LI, Zeli LUO, Manli ZHANG, Juan WU, Yang SHI

著者情報

Zhangcheng LI
1) Department of Veterinary Medicine, Rongchang Campus of Southwest University, Chongqing 402460, China
Fangjun CHENG
1) Department of Veterinary Medicine, Rongchang Campus of Southwest University, Chongqing 402460, China
2) Chongqing Engineering Research Center of Veterinary Science, Chongqing 402460, China
Shimei LAN
1) Department of Veterinary Medicine, Rongchang Campus of Southwest University, Chongqing 402460, China
Jianhua GUO
1) Department of Veterinary Medicine, Rongchang Campus of Southwest University, Chongqing 402460, China
2) Chongqing Engineering Research Center of Veterinary Science, Chongqing 402460, China
Wei LIU
1) Department of Veterinary Medicine, Rongchang Campus of Southwest University, Chongqing 402460, China
Xiaoyan LI
1) Department of Veterinary Medicine, Rongchang Campus of Southwest University, Chongqing 402460, China
Zeli LUO
1) Department of Veterinary Medicine, Rongchang Campus of Southwest University, Chongqing 402460, China
Manli ZHANG
1) Department of Veterinary Medicine, Rongchang Campus of Southwest University, Chongqing 402460, China
Juan WU
1) Department of Veterinary Medicine, Rongchang Campus of Southwest University, Chongqing 402460, China
Yang SHI
1) Department of Veterinary Medicine, Rongchang Campus of Southwest University, Chongqing 402460, China

キーワード: epidemiology, MLST, Pasteurella multocida, poultry

ジャーナルフリー

2018 年 80 巻 6 号 p. 921-929

DOI https://doi.org/10.1292/jvms.18-0049

Browse “Advance online publication” version

詳細

抄録

Fowl cholera caused by Pasteurella multocida has always been a disease of global importance for poultry production. The aim of this study was to obtain more information about the epidemiology of avian P. multocida infection in southwest China and the genetic characteristics of clinical isolates. P. multocida isolates were characterized by biochemical and molecular-biological methods. The distributions of the capsular serogroups, the phenotypic antimicrobial resistance profiles, lipopolysaccharide (LPS) genotyping and the presence of 19 virulence genes were investigated in 45 isolates of P. multocida that were associated with clinical disease in poultry. The genetic diversity of P. multocida strains was performed by 16S rRNA and rpoB gene sequence analysis as well as multilocus sequence typing (MLST). The results showed that most (80.0%) of the P. multocida isolates in this study represented special P. multocida subspecies, and 71.1% of the isolates showed multiple-drug resistance. 45 isolates belonged to capsular types: A (100%) and two LPS genotypes: L1 (95.6%) and L3 (4.4%). MLST revealed two new alleles (pmi77 and gdh57) and one new sequence type (ST342). ST129 types dominated in 45 P. multocida isolates. Isolates belonging to ST129 were with the genes ompH+plpB+ptfA+tonB, whereas ST342 included isolates with fur+hgbA+tonB genes. Population genetic analysis and the MLST results revealed that at least one new ST genotype was present in the avian P. multocida in China. These findings provide novel insights into the epidemiological characteristics of avian P. multocida isolates in southwest China.

責任著者(Corresponding author)

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