Statistical analysis of the usefulness of follicle-sinus complexes as a novel          diagnostic material for canine rabies

Nozomi SHIWA; Hiroko YAMASHITA; Koki TOMIOKA; Kazunori KIMITSUKI; Daria Llenaresas MANALO; Satoshi INOUE; Chun-Ho PARK

doi:10.1292/jvms.18-0591

Pathology

Statistical analysis of the usefulness of follicle-sinus complexes as a novel diagnostic material for canine rabies

Nozomi SHIWA, Hiroko YAMASHITA, Koki TOMIOKA, Kazunori KIMITSUKI, Daria Llenaresas MANALO, Satoshi INOUE, Chun-Ho PARK

Author information

Nozomi SHIWA
Department of Veterinary Pathology, School of Veterinary Medicine, Kitasato University, 23-35-1, Higashi, Towada, Aomori 034-8628, Japan
Hiroko YAMASHITA
Department of Veterinary Pathology, School of Veterinary Medicine, Kitasato University, 23-35-1, Higashi, Towada, Aomori 034-8628, Japan
Koki TOMIOKA
Department of Veterinary Pathology, School of Veterinary Medicine, Kitasato University, 23-35-1, Higashi, Towada, Aomori 034-8628, Japan
Kazunori KIMITSUKI
Department of Microbiology, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita 879-5593, Japan
Daria Llenaresas MANALO
Veterinary Research Department, Research Institute for Tropical Medicine, Department of Health, 9002 Research Drive, Filinvest Corporate City, Alabang, Muntinlupa City 1781, Philippines
Satoshi INOUE
Department of Veterinary Science, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan
Chun-Ho PARK
Department of Veterinary Pathology, School of Veterinary Medicine, Kitasato University, 23-35-1, Higashi, Towada, Aomori 034-8628, Japan

Keywords: Follicle-sinus complex, FSC, post-mortem diagnosis, rabies

JOURNAL FREE ACCESS

2019 Volume 81 Issue 2 Pages 182-185

DOI https://doi.org/10.1292/jvms.18-0591

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Details

Abstract

In the present study, follicle-sinus complexes (FSCs) were harvested from the muzzle skin of 123 dogs with suspected canine rabies, and the sensitivity and specificity of FSC analysis were compared with those of brain tissue immunohistochemistry analysis. In the FSCs, viral antigen was detected from Merkel cells. Sensitivity was 97.3%, specificity was 100%, and the coefficient κ was 0.88. These results reconfirm that FSCs are very useful for the postmortem diagnosis of canine rabies, and suggest that 5 FSCs can yield results that are almost equivalent to those derived from brain tissue analysis in rabid dogs.

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