Development and evaluation of an enzyme-linked immunosorbent assay based on recombinant TgSRS2 for serodiagnosis of Toxoplasma gondii infection in cats

Yang GAO; Huanping GUO; Paul Franck Adjou MOUMOUNI; Mingming LIU; Jixu LI; Artemis EFSTRATIOU; Eloiza May GALON; Rochelle Haidee YBANEZ; Tatsunori MASATANI; Takehisa SOMA; Xuenan XUAN

doi:10.1292/jvms.20-0231

Parasitology

Development and evaluation of an enzyme-linked immunosorbent assay based on recombinant TgSRS2 for serodiagnosis of Toxoplasma gondii infection in cats

Yang GAO, Huanping GUO, Paul Franck Adjou MOUMOUNI, Mingming LIU, Jixu LI, Artemis EFSTRATIOU, Eloiza May GALON, Rochelle Haidee YBANEZ, Tatsunori MASATANI, Takehisa SOMA, Xuenan XUAN

Author information

Yang GAO
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
Huanping GUO
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
Paul Franck Adjou MOUMOUNI
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
Mingming LIU
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
Jixu LI
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
Artemis EFSTRATIOU
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
Eloiza May GALON
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
Rochelle Haidee YBANEZ
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
Tatsunori MASATANI
Transboundary Animal Diseases Research Center, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kogoshima 890-0065, Japan
Takehisa SOMA
Veterinary Diagnostic Laboratory, Marupi Lifetech Co., Ltd., Fushio-cho, Ikeda, Osaka 563-0011, Japan
Xuenan XUAN
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan

Keywords: cat, enzyme-linked immunosorbent assay, serodiagnosis, SAG1-related sequence 2 of Toxoplasma gondii, toxoplasmosis

JOURNAL OPEN ACCESS

2020 Volume 82 Issue 11 Pages 1662-1665

DOI https://doi.org/10.1292/jvms.20-0231

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Abstract

An enzyme-linked immunosorbent assay (ELISA) based on recombinant SAG1-related sequence 2 of Toxoplasma gondii (rTgSRS2) was developed to detect toxoplasmosis in cats. The specificity and sensitivity of rTgSRS2 ELISA were confirmed using a series of serum samples from T. gondii-experimentally infected mice. A total of 76 field samples from cats were examined by the developed ELISA. The rTgSRS2 ELISA showed a good diagnostic performance characterized by high concordance (88.16) and kappa value (0.76) with latex agglutination test (LAT). The sensitivity and specificity of the test were 92.68% and 82.86%, respectively. These results suggest that the ELISA based on rTgSRS2 could be a useful tool for serodiagnosis of T. gondii infection in cats.

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