Molecular characterization of Brucella ceti from a          bottlenose dolphin (Tursiops truncatus) with osteomyelitis in the western          Pacific

Yuichi UENO; Makio YANAGISAWA; Sayuri KINO; Satoru SHIGENO; Makoto OSAKI; Daisuke TAKAMATSU; Ken KATSUDA; Tadashi MARUYAMA; Kazue OHISHI

doi:10.1292/jvms.20-0015

Bacteriology

Molecular characterization of Brucella ceti from a bottlenose dolphin (Tursiops truncatus) with osteomyelitis in the western Pacific

Yuichi UENO, Makio YANAGISAWA, Sayuri KINO, Satoru SHIGENO, Makoto OSAKI, Daisuke TAKAMATSU, Ken KATSUDA, Tadashi MARUYAMA, Kazue OHISHI

Author information

Yuichi UENO
Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan
Makio YANAGISAWA
Okinawa Churashima Foundation, 888 Ishikawa, Kunigamigun Motobucho, Okinawa 905-0214, Japan
Sayuri KINO
Okinawa Churashima Foundation, 888 Ishikawa, Kunigamigun Motobucho, Okinawa 905-0214, Japan
Satoru SHIGENO
Okinawa Prefectural Institute of Animal Health, 3-1 Kenekadan, Uruma, Okinawa 904-2241, Japan
Makoto OSAKI
Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan
Daisuke TAKAMATSU
Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan
The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagito, Gifu, Gifu 501-1193, Japan
Ken KATSUDA
Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan
Tadashi MARUYAMA
School of Marine Biosciences, Kitasato University, 1-15-1 Kitasato, Minami Ward, Sagamihara, Kanagawa 252-0373, Japan
Kazue OHISHI
School of Engineering, Tokyo Polytechnic University, 1583 Iiyama, Atsugi, Kanagawa 243-0297, Japan

Keywords: bottlenose dolphin, Brucella ceti, brucellosis, molecular characterization, western Pacific

JOURNAL OPEN ACCESS
Supplementary material

2020 Volume 82 Issue 6 Pages 754-758

DOI https://doi.org/10.1292/jvms.20-0015

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Abstract

Although the presence of Brucella spp. in the western Pacific has been suggested by epidemiological studies on cetaceans, it has not been confirmed by bacterial isolation. Here, for the first time, we report that a marine Brucella strain was isolated in the western Pacific from a bottlenose dolphin with osteomyelitis. The isolate from the lesion was confirmed to be B. ceti of sequence type 27 by multilocus sequence typing and Bruce-ladder PCR. Infrequent-restriction-site PCR and omp2 gene sequencing revealed that molecular characteristics of this isolate were similar to those of Brucella DNA previously detected from minke whales in the western North Pacific. These results suggest that genetically related Brucella strains circulate in cetacean species in this region.

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