Effects of zinc on tissue uptake and toxicity of lead in Sprague Dawley rat

Andrew KATABA; Shouta M.M. NAKAYAMA; Yared Beyene YOHANNES; Haruya TOYOMAKI; Hokuto NAKATA; Yoshinori IKENAKA; Mayumi ISHIZUKA

doi:10.1292/jvms.20-0684

Toxicology

Effects of zinc on tissue uptake and toxicity of lead in Sprague Dawley rat

Andrew KATABA, Shouta M.M. NAKAYAMA, Yared Beyene YOHANNES, Haruya TOYOMAKI, Hokuto NAKATA, Yoshinori IKENAKA, Mayumi ISHIZUKA

著者情報

Andrew KATABA
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita-Ku, Sapporo, Hokkaido 060-0818, Japan
Department of Biomedical Sciences, School of Veterinary Medicine, The University of Zambia, P.O. Box 32379, Lusaka, Zambia
Shouta M.M. NAKAYAMA
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita-Ku, Sapporo, Hokkaido 060-0818, Japan
Yared Beyene YOHANNES
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita-Ku, Sapporo, Hokkaido 060-0818, Japan
Department of Chemistry, College of Natural and Computational Science, University of Gondar, P.O. Box 196, Gondar, Ethiopia
Haruya TOYOMAKI
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita-Ku, Sapporo, Hokkaido 060-0818, Japan
Hokuto NAKATA
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita-Ku, Sapporo, Hokkaido 060-0818, Japan
Yoshinori IKENAKA
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita-Ku, Sapporo, Hokkaido 060-0818, Japan
Water Research Group, School of Environmental Sciences and Development, North-West University, Private Bag X6001, Potchefstroom, South Africa
Mayumi ISHIZUKA
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita-Ku, Sapporo, Hokkaido 060-0818, Japan

キーワード: lead, oxidative stress, toxicity, zinc co-administration

ジャーナルオープンアクセス

2021 年 83 巻 11 号 p. 1674-1685

DOI https://doi.org/10.1292/jvms.20-0684

Browse “Advance online publication” version

詳細

抄録

Lead (Pb) exposure occurs together with other metals including zinc (Zn). This study investigated the impact of Zn on Pb tissue accumulation and Pb-induced toxicities. Animals (n=6 rats per group) were exposed to lead acetate (PbAc) or a combination of PbAc and zinc acetate (ZnAc) under the following groups: control (deionized water), low PbAc [12 mg/kg PbAc (3 mg PbAc/rat/day)], low PbAc–ZnAc [12 mg/kg PbAc (3 mg PbAc/rat/day) + 0.2 mg ZnAc/rat/48 hr], high PbAc [120 mg/kg (30 mg PbAc/rat/day)], and high PbAc–ZnAc [120 mg/kg (30 mg PbAc/rat/day) + 1 mg ZnAc/rat/48 hr] for 8 weeks. A significant reduction in body weight gain was observed in the high PbAc group relative to the control group. Muscles and testes both had reduced and increased Pb uptake in low PbAc–ZnAc and high PbAc–ZnAc groups compared to PbAc only groups, respectively. Bone Pb levels in the high PbAc–ZnAc group were lower than the high PbAc group. Zinc co-administration attenuated Pb-induced inhibition of delta aminolaevulinic acid dehydratase enzyme and enhanced catalase enzyme activity at a high level of exposure. Moreover, ZnAc seems to have minimized the effects of Pb-induced mRNA dysregulation in antioxidant and antiapoptotic enzymes encoding genes. Heme oxygenase-1 was downregulated in the kidney and brain in the low PbAc group. Liver glutathione peroxidase and thioredoxin reductase-1 were downregulated in the high PbAc group. These findings suggest that zinc co-administration with lead may partially mitigate against Pb-induced toxicities.

責任著者(Corresponding author)

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