Monitoring of chronic wasting disease using real-time quaking-induced conversion assay in Japan

Akio SUZUKI; Kazuhei SAWADA; Temuulen ERDENEBAT; Takeshi YAMASAKI; Minoru TOBIUME; Kinuyo SUGA; Motohiro HORIUCHI

doi:10.1292/jvms.21-0368

Public Health

Monitoring of chronic wasting disease using real-time quaking-induced conversion assay in Japan

Akio SUZUKI, Kazuhei SAWADA, Temuulen ERDENEBAT, Takeshi YAMASAKI, Minoru TOBIUME, Kinuyo SUGA, Motohiro HORIUCHI

Author information

Akio SUZUKI
Laboratory of Veterinary Hygiene, Faculty of Veterinary Medicine, Graduate School of Infectious Diseases, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan
Kazuhei SAWADA
Laboratory of Veterinary Hygiene, Faculty of Veterinary Medicine, Graduate School of Infectious Diseases, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan
Temuulen ERDENEBAT
Laboratory of Veterinary Hygiene, Faculty of Veterinary Medicine, Graduate School of Infectious Diseases, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan
Takeshi YAMASAKI
Biomedical Animal Research Laboratory, Institute for Genetic Medicine, Hokkaido University, Kita 15, Nishi 9, Kita-ku, Sapporo, Hokkaido 060-0815, Japan
Minoru TOBIUME
Department of Pathology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan
Kinuyo SUGA
Hyogo Prefecture Nishiharima Meat Hygiene Inspection Office, Shingu-cho, Tatsuno, Hyogo 679-4322, Japan
Motohiro HORIUCHI
Laboratory of Veterinary Hygiene, Faculty of Veterinary Medicine, Graduate School of Infectious Diseases, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan
Global Station for Zoonosis Control, Global Institute for Collaborative Research and Education, Hokkaido University, Kita 20, Nishi 10, Kita-ku, Sapporo, Hokkaido 001-0020, Japan

Keywords: chronic wasting disease, prion, prion protein, real-time quaking-induced conversion, scrapie

JOURNAL OPEN ACCESS

2021 Volume 83 Issue 11 Pages 1735-1739

DOI https://doi.org/10.1292/jvms.21-0368

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Abstract

There has been no report on Chronic wasting disease (CWD) cases in Japan to date; however, there is concern about the geographic spread of CWD. To clarify the CWD status in Japan, we conducted CWD monitoring using real-time quaking-induced conversion (RT-QuIC) assay which can detect the low level of CWD prions. A total of 690 obex samples collected from sika deer and Reeves’s muntjac in Hokkaido and Honshu was tested for CWD prions. No CWD-positive cases were found, suggesting that CWD is nonexistent in Japan. Our results also indicate that RT-QuIC assay is useful for continuous monitoring of CWD. Furthermore, nucleotide sequence analysis of the PrP gene revealed sika deer in Japan harbor CWD susceptible allele.

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