Application of a real-time PCR assay for the detection of Ascaris            suum DNA in the liver of experimentally infected chickens

Yen Thi Hoang NGUYEN; Nariaki NONAKA; Haruhiko MARUYAMA; Ayako YOSHIDA

doi:10.1292/jvms.20-0404

Parasitology

Application of a real-time PCR assay for the detection of Ascaris suum DNA in the liver of experimentally infected chickens

Yen Thi Hoang NGUYEN, Nariaki NONAKA, Haruhiko MARUYAMA, Ayako YOSHIDA

Author information

Yen Thi Hoang NGUYEN
Laboratory of Veterinary Parasitic Diseases, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen-Kibanadai Nishi, Miyazaki 889-2192, Japan
Department of Parasitology, Faculty of Veterinary Medicine, Vietnam National University of Agriculture, Trau Quy, Gia Lam, Ha Noi 100000, Vietnam
Nariaki NONAKA
Laboratory of Veterinary Parasitic Diseases, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen-Kibanadai Nishi, Miyazaki 889-2192, Japan
Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan
Haruhiko MARUYAMA
Division of Parasitology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake-cho, Miyazaki 889-1692, Japan
Ayako YOSHIDA
Laboratory of Veterinary Parasitic Diseases, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen-Kibanadai Nishi, Miyazaki 889-2192, Japan
Centre for Animal Disease Control (CADIC), University of Miyazaki, 1-1 Gakuen-Kibanadai Nishi, Miyazaki 889-2192, Japan

Keywords: Ascaris suum, chicken liver, real-time PCR assay, white spot lesion

JOURNAL OPEN ACCESS

2021 Volume 83 Issue 4 Pages 671-674

DOI https://doi.org/10.1292/jvms.20-0404

Browse “Advance online publication” version

Details

Abstract

This study aimed to evaluate the sampling method for the detection of Ascaris suum larval DNA in chicken livers using real-time PCR. Chickens were inoculated with A. suum eggs of a single dose (Group A) or repeatedly low doses (Group B). White spots (WSs) were continuously observed on liver from day 3 after the last infection in Group B and day 14 in Group A. In Group A, larval DNA was detected in WS lesions (78.6%) at a significantly higher rate than in the remaining tissue samples (31.3%). In conclusion, applying WS lesions to the assay improved the detection rate of A. suum DNA in chicken livers, especially in the case of a single infection.

Corresponding author

Register with J-STAGE for free!