Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis

Yuta KINOSHITA; Hironaga KAKOI; Taichiro ISHIGE; Takashi YAMANAKA; Hidekazu NIWA; Eri UCHIDA-FUJII; Toshio NUKADA; Takanori UENO

doi:10.1292/jvms.21-0539

Bacteriology

Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis

Yuta KINOSHITA, Hironaga KAKOI, Taichiro ISHIGE, Takashi YAMANAKA, Hidekazu NIWA, Eri UCHIDA-FUJII, Toshio NUKADA, Takanori UENO

Author information

Yuta KINOSHITA
Microbiology Division, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan
Hironaga KAKOI
Genetic Analysis Department, Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan
Taichiro ISHIGE
Genetic Analysis Department, Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan
Takashi YAMANAKA
Epizootic Prevention Section, Equine Department, Japan Racing Association, 1-1-1 Nishi Shinnbashi, Minato-ku, Tokyo 105-0003, Japan
Hidekazu NIWA
Microbiology Division, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan
Eri UCHIDA-FUJII
Microbiology Division, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan
Toshio NUKADA
Microbiology Division, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan
Takanori UENO
Microbiology Division, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan

Keywords: contagious equine metritis, nucleic acid amplification test, real-time PCR, Taylorella equigenitalis

JOURNAL OPEN ACCESS
Supplementary material

2022 Volume 84 Issue 1 Pages 129-132

DOI https://doi.org/10.1292/jvms.21-0539

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Abstract

Taylorella equigenitalis causes contagious equine metritis. Here we compared seven nucleic acid amplification tests for T. equigenitalis to select a rapid and reliable diagnostic method. The 95% detection limits of each assay varied greatly: real-time PCR had the lowest detection limit (0.77 fg/reaction); those of some of the conventional PCRs (cPCRs) were >100 fg/reaction. In experimentally infected samples, real-time PCR and semi-nested PCR showed the highest positive numbers (33 out of 42 samples), but two of the cPCRs detected only 2 and 7 positive results. Our results indicate that the use of sensitive molecular assays is important for the efficient detection of T. equigenitalis in clinical samples.

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