Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250
Toxicology
Elucidation of the neurological effects of clothianidin exposure at the no-observed-adverse-effect level (NOAEL) using two-photon microscopy in vivo imaging
Misaki NISHIShouta SUGIOTetsushi HIRANODaisuke KATOHiroaki WAKEAsuka SHODAMidori MURATAYoshinori IKENAKAYoshiaki TABUCHIYouhei MANTANIToshifumi YOKOYAMANobuhiko HOSHI
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JOURNAL OPEN ACCESS

2022 Volume 84 Issue 4 Pages 585-592

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Abstract

Neonicotinoid pesticides (NNs) cause behavioral abnormalities in mammals, raising concerns about their effects on neural circuit activity. We herein examined the neurological effects of the NN clothianidin (CLO) by in vivo Ca2+ imaging using two-photon microscopy. Mice were fed the no-observed-adverse-effect-level (NOAEL) dose of CLO for 2 weeks and their neuronal activity in the primary somatosensory cortex (S1) was observed weekly for 2 weeks. CLO exposure caused a sustained influx of Ca2+ in neurons in the S1 2/3 layers, indicating hyperactivation of neurons. In addition, microarray gene expression analysis suggested the induction of neuroinflammation and changes in synaptic activity. These results demonstrate that exposure to the NOAEL dose of CLO can overactivate neurons and disrupt neuronal homeostasis.

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https://creativecommons.org/licenses/by-nc-nd/4.0/
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