Novel recombinant porcine enterovirus G viruses lacking structural proteins          are maintained in pig farms in Japan

Ryo IMAI; Wen RONGDUO; Li KAIXIN; Sumiya BORJIGIN; Hirofumi MATSUMURA; Tsuneyuki MASUDA; Takuji OZAWA; Mami OBA; Shinji MAKINO; Makoto NAGAI; Tetsuya MIZUTANI

doi:10.1292/jvms.22-0505

Virology

Novel recombinant porcine enterovirus G viruses lacking structural proteins are maintained in pig farms in Japan

Ryo IMAI, Wen RONGDUO, Li KAIXIN, Sumiya BORJIGIN, Hirofumi MATSUMURA, Tsuneyuki MASUDA, Takuji OZAWA, Mami OBA, Shinji MAKINO, Makoto NAGAI, Tetsuya MIZUTANI

著者情報

Ryo IMAI
Center for Infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo, Japan
Graduate School of Agriculture Cooperative Division of Veterinary Science, Tokyo University of Agriculture and Technology, Tokyo, Japan
Wen RONGDUO
Center for Infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo, Japan
Graduate School of Agriculture Cooperative Division of Veterinary Science, Tokyo University of Agriculture and Technology, Tokyo, Japan
Li KAIXIN
Center for Infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo, Japan
Sumiya BORJIGIN
Center for Infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo, Japan
Hirofumi MATSUMURA
Center for Infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo, Japan
Tsuneyuki MASUDA
Kurayoshi Livestock Hygiene Service Center, Tottori, Japan
Takuji OZAWA
Japanese Animal Hospital Association, Tokyo, Japan
Mami OBA
Center for Infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo, Japan
Shinji MAKINO
Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, TX, USA
Makoto NAGAI
Laboratory of Infectious Diseases, Department of Veterinary Medicine, Faculty of Veterinary Medicine, Azabu University, Kanagawa, Japan
Tetsuya MIZUTANI
Center for Infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo, Japan
Graduate School of Agriculture Cooperative Division of Veterinary Science, Tokyo University of Agriculture and Technology, Tokyo, Japan

キーワード: baculoviral inhibitor of apoptosis (IAP) repeat, genomic recombination, papain-like cysteine protease, porcine enterovirus G, porcine toro virus

ジャーナルオープンアクセス
電子付録

2023 年 85 巻 2 号 p. 252-265

DOI https://doi.org/10.1292/jvms.22-0505

Browse “Advance online publication” version

詳細

抄録

Type 1 recombinant enterovirus G (EV-G), which carries the papain-like cysteine protease (PLCP) gene of torovirus between its 2C/3A regions, and type 2 recombinant EV-G, which carries the torovirus PLCP gene with its flanking regions having non-EV-G sequences in place of the viral structural genes, have been detected in pig farms in several countries. In a previous study, we collected 222 fecal samples from 77 pig farms from 2104 to 2016 and detected one type 2 recombinant EV-G genome by metagenomics sequencing. In this study, we reanalyzed the metagenomic data and detected 11 type 2 recombinant EV-G genomes. In addition, we discovered new type 2 recombinant EV-G genomes of the two strains from two pig farms samples in 2018 and 2019. Thus, we identified the genomes of 13 novel type 2 recombinant EV-Gs isolated from several pig farms in Japan. Type 2 recombinant EV-G has previously been detected only in neonatal piglets. The present findings suggest that type 2 recombinant EV-G replicates in weaning piglets and sows. The detection of type 1 recombinant EV-Gs and type 2 recombinant EV-Gs at 3-year and 2-year intervals, respectively, from the same pig farm suggests that the viruses were persistently infecting or circulating in these farms.

責任著者(Corresponding author)

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