Grid1 regulates the onset of puberty in female rats

Jing YE; Xiaoqian LI; Zhihao PAN; Zhuoya WU; Yanyun ZHU; Wei ZHANG; Juntai LU; Shuangshuang XU; Ping QIN; Ya LIU; Yunsheng LI; Yinghui LING; Fugui FANG

doi:10.1292/jvms.23-0208

Physiology

Grid1 regulates the onset of puberty in female rats

Jing YE, Xiaoqian LI, Zhihao PAN, Zhuoya WU, Yanyun ZHU, Wei ZHANG, Juntai LU, Shuangshuang XU, Ping QIN, Ya LIU, Yunsheng LI, Yinghui LING, Fugui FANG

Author information

Jing YE
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Anhui, China
Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, Anhui, China
Xiaoqian LI
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Anhui, China
Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, Anhui, China
Zhihao PAN
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Anhui, China
Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, Anhui, China
Zhuoya WU
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Anhui, China
Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, Anhui, China
Yanyun ZHU
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Anhui, China
Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, Anhui, China
Wei ZHANG
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Anhui, China
Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, Anhui, China
Juntai LU
Anhui Provincial Key Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui, China
Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, Anhui, China
Shuangshuang XU
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Anhui, China
Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, Anhui, China
Ping QIN
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Anhui, China
Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, Anhui, China
Ya LIU
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Anhui, China
Anhui Provincial Key Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui, China
Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, Anhui, China
Yunsheng LI
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Anhui, China
Anhui Provincial Key Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui, China
Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, Anhui, China
Yinghui LING
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Anhui, China
Anhui Provincial Key Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui, China
Fugui FANG
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Anhui, China
Anhui Provincial Key Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui, China
Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, Anhui, China

Keywords: Grid1, puberty, rat, reproductive performance

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Supplementary material

2024 Volume 86 Issue 5 Pages 497-506

DOI https://doi.org/10.1292/jvms.23-0208

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Abstract

The study aimed to investigate the effect of Grid1, encoding the glutamate ionotropic receptor delta type subunit 1 (GluD1), on puberty onset in female rats. Grid1 mRNA and protein expression was detected in the hypothalamus of female rats at prepuberty and puberty. The levels of Grid1 mRNA in the hypothalamus, the fluorescence intensity in the arcuate nucleus and paraventricular nucleus of the prepubertal rats was significantly lower than pubertal. Additionally, the expression of Grid1 was suppressed in primary hypothalamus cells and prepubertal rat. Finally, investigated the effect of Grid1 knockdown on puberty onset and reproductive performance. Treatment of hypothalamic neurons with LV-Grid1 decreased the level of Grid1 and Rfrp-3 (encoding RFamide-related peptide 3) mRNA expression, but increased the Gnrh (encoding gonadotropin-releasing hormone) mRNA levels. After an ICV injection, the time for the rat vaginal opening occurred earlier. Moreover, Gnrh mRNA expression was increased, whereas Rfrp-3 mRNA expression was decreased in the hypothalamus. The concentration of progesterone (P₄) in the serum was significantly decreased compare with control group. Ovary hematoxylin-eosin staining revealed that the LV-Grid1 group mainly contained primary and secondary follicles. The reproductive performance of the rats was not affected by the Grid1 knockdown. Therefore, Grid1 may aﬀect the onset of puberty in female rats by regulating the levels of Gnrh, and Rfrp-3 in the hypothalamus, as well as the concentrations of P₄, but not reproduction performance.

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