Monoclonal proliferation of B-cells with two integration sites of bovine          leukemia virus proviral DNA in cattle with enzootic bovine leukosis

Masaki MAEZAWA; Shiho TAKEZAWA; Kana SAKAGUCHI-MAEZAWA; Yusuke TANAKA; Ken-ichi WATANABE; Yoshiyasu KOBAYASHI; Hisashi INOKUMA

doi:10.1292/jvms.24-0037

Virology

Monoclonal proliferation of B-cells with two integration sites of bovine leukemia virus proviral DNA in cattle with enzootic bovine leukosis

Masaki MAEZAWA, Shiho TAKEZAWA, Kana SAKAGUCHI-MAEZAWA, Yusuke TANAKA, Ken-ichi WATANABE, Yoshiyasu KOBAYASHI, Hisashi INOKUMA

Author information

Masaki MAEZAWA
Laboratory of OSG Veterinary Science for Global Disease Management, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
Shiho TAKEZAWA
Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
Kana SAKAGUCHI-MAEZAWA
Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
Yusuke TANAKA
Department of Functional Anatomy and Neuroscience, Asahikawa Medical University, Hokkaido, Japan
Ken-ichi WATANABE
Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
Yoshiyasu KOBAYASHI
Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
Hisashi INOKUMA
Laboratory of OSG Veterinary Science for Global Disease Management, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
Laboratory of Farm Animal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan

Keywords: bovine leukemia virus, clonality, enzootic bovine leukosis, superinfection

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Supplementary material

2024 Volume 86 Issue 6 Pages 653-655

DOI https://doi.org/10.1292/jvms.24-0037

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Details

Abstract

The present study analyzed B-cell clonality and bovine leukemia virus (BLV) provirus integration sites in cattle with enzootic bovine leukosis (EBL) having BLV proviral copy numbers less or greater than the number of bovine nucleated cells. EBL cattle with BLV copy numbers less than the number of bovine nucleated cells showed monoclonal and biclonal proliferation of B-cells with one BLV provirus integration site. On the other hand, EBL cattle with BLV copy numbers greater than the number of bovine nucleated cells showed monoclonal proliferation of B-cells with two BLV provirus integration sites. These results suggest that superinfection of BLV can occur in EBL cattle.

Corresponding author

Funder information

1.Fund name: Japan Society for the Promotion of Science

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