Molecular Cloning and Expression Analysis of the Bat Toll-Like Receptor 3,7, and 9

Koichiro IHA; Tsutomu OMATSU; Shumpei WATANABE; Naoya UEDA; Satoshi TANIGUCHI; Hikaru FUJII; Yoshiyuki ISHII; Shigeru KYUWA; Hiroomi AKASHI; Yasuhiro YOSHIKAWA

doi:10.1292/jvms.09-0050

This article has now been updated. Please use the final version.

Molecular Cloning and Expression Analysis of the Bat Toll-Like Receptor 3,7, and 9

Koichiro IHA, Tsutomu OMATSU, Shumpei WATANABE, Naoya UEDA, Satoshi TANIGUCHI, Hikaru FUJII, Yoshiyuki ISHII, Shigeru KYUWA, Hiroomi AKASHI, Yasuhiro YOSHIKAWA

Author information

Koichiro IHA
Department of Biomedical Science, The University of Tokyo
Tsutomu OMATSU
Laboratory of Vector-borne Viruses, National Institute of Infectious Diseases
Shumpei WATANABE
Department of Veterinary Microbiology, The University of Tokyo
Naoya UEDA
Department of Biomedical Science, The University of Tokyo
Satoshi TANIGUCHI
Department of Biomedical Science, The University of Tokyo
Hikaru FUJII
Department of Veterinary Microbiology, The University of Tokyo
Yoshiyuki ISHII
Department of Biomedical Science, The University of Tokyo
Shigeru KYUWA
Department of Biomedical Science, The University of Tokyo
Hiroomi AKASHI
Department of Veterinary Microbiology, The University of Tokyo
Yasuhiro YOSHIKAWA
Department of Biomedical Science, The University of Tokyo

Keywords: chiroptera, expression analysis, molecular cloning, sequencing, toll-like receptor

JOURNAL FREE ACCESS Advance online publication

Article ID: 09-0050

DOI https://doi.org/10.1292/jvms.09-0050

The final version of this article is now available: Vol. 72 (2010) , No. 2 p.217

Details

Abstract

In this study, cDNA of Toll-like receptor (TLR) 3, 7, and 9 were synthesized and completely sequenced. The coding regions of cDNA for bat TLR3, 7, and 9 were 2,718, 3,150, and 3,090 bp in length, respectively. Open reading frames encoded 905, 1,049, and 1,029 amino acids for TLR3, 7, and 9, respectively. Nucleotide sequences, predicted amino acid sequences and predicted domain structures of the three bat TLRs had high homology with those of other mammals. In addition, the expression profile of each TLR in main organs was analyzed. Expression of TLR3 was highest in liver, whereas TLR7 and TLR9 expression was highest in spleen.

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