Cytochrome P450 1A Dependent Activities in Deer, Cattle, and Horses

Wageh Sobhy DARWISH; Yoshinori IKENAKA; Elsaid Abozeid ELDALY; Marumi OHNO; Kentaro Q. SAKAMOTO; Shoichi FUJITA; Mayumi ISHIZUKA

doi:10.1292/jvms.09-0318

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Cytochrome P450 1A Dependent Activities in Deer, Cattle, and Horses

Wageh Sobhy DARWISH, Yoshinori IKENAKA, Elsaid Abozeid ELDALY, Marumi OHNO, Kentaro Q. SAKAMOTO, Shoichi FUJITA, Mayumi ISHIZUKA

著者情報

キーワード: CYP1A1, cytochrome P450, ethoxyresorufin O-deethylase, horse, ungulate

ジャーナルフリー早期公開

論文ID: 09-0318

DOI https://doi.org/10.1292/jvms.09-0318

この記事には本公開記事があります。

The final version of this article is now available: Vol. 72 (2010) , No. 5 p.561

詳細

抄録

The objective of this study was to investigate and characterize the metabolic activities of CYP1A in deer, cattle, and horses in a comparison to those of rats using ethoxyresorufin O-deethylation (EROD) and methoxyresorufin O-demethylation (MROD) assays. We performed an inhibition study for these activities using anti-rat CYP1A1 antibody, and identified that these activities were due to CYP1A subfamily. Inter-species difference in the CYP1A dependent activities was highly observed in this study. Especially, we found that the horse had the highest EROD and MROD activities among the examined animal species in this study. In the kinetic analysis, horse showed the highest Vmax and catalytic efficiency (Vmax/Km) followed by cattle, deer and finally rats.

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