Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250

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Isolation of Trypanosoma (Megatrypanum) theileri from Dairy Cattle in Taiwan
Yen-Feng LEEChing-Chang CHENGNai-Nu LINShih-An LIUKwong-Chung TUNGYung-Tsung CHIU
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JOURNAL FREE ACCESS Advance online publication

Article ID: 09-0343

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Abstract
Although Trypanosoma (Megatrypanum) theileri, a blood parasite of Bovidae species, is spread widely throughout the world, it has never been reported in Taiwan. An anti-coagulated blood sample from febrile dairy cattle was directly smeared and no parasite was observed. However, a highly distinctive morphological feature of trypanosome appeared in baby hamster kidney (BHK) cell culture inoculated with non-thrown blood buffy coat. The different stages and typical ultrastructures of trypanosome were observed in our isolate. The isolate was subsequently identified as T. theileri by species-specific PCR assay (Tth625), 18S RNA sequencing alignment and internal transcribed spacer of ribosomal genes (ITS) as a marker for molecular phylogenetic analysis. Intriguingly, the first T. theileri isolate in Taiwan (TWTth1) can be periodicaly passaged in BHK cell culture for more than one year and retain good re-cryopreservation viability. The BHK culture method would excellent for diagnostic isolation, sustaining long-term development, life cycle or further studies.
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© 2010 by the Japanese Society of Veterinary Science

この記事はクリエイティブ・コモンズ [表示 - 非営利 - 改変禁止 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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