Ethyl Pyruvate Down-Regulates Tumor Necrosis Factor Alpha, Interleukin (IL)-6 and Up-Regulates IL-10 in Lipopolysaccharide-Stimulated Canine Peripheral Blood Mononuclear Cells

Abstract

We investigated the anti-inflammatory effects of ethyl pyruvate (EP) on LPS-stimulated canine PBMCs in vitro. We found that EP treatment inhibited the mRNA expressions of pro-inflammatory cytokines (TNF-α and IL-6), but induced mRNA expression of anti-inflammatory cytokines (IL-10). ELISA measurements revealed that EP also effectively down-regulates the LPS-induced increase in pro-inflammatory cytokine release, while up-regulating anti-inflammatory cytokine release. These data indicate that EP could be an effective anti-inflammatory agent in dogs.