Characterization of Genes Encoding Virulence Determinants and Toxins in Staphylococcus aureus from Bovine Milk in Central Italy

Patrizia Casagrande PROIETTI; Giacomo COPPOLA; Annalisa BIETTA; Maria Luisa MARENZONI; Doreene R. HYATT; Mauro COLETTI; Fabrizio PASSAMONTI

doi:10.1292/jvms.10-0158

This article has now been updated. Please use the final version.

Characterization of Genes Encoding Virulence Determinants and Toxins in Staphylococcus aureus from Bovine Milk in Central Italy

Patrizia Casagrande PROIETTI, Giacomo COPPOLA, Annalisa BIETTA, Maria Luisa MARENZONI, Doreene R. HYATT, Mauro COLETTI, Fabrizio PASSAMONTI

Author information

Patrizia Casagrande PROIETTI
Department of Biopathological Science and Hygiene of Food and Animal Production, Faculty of Veterinary Medicine, University of Perugia
Giacomo COPPOLA
Pathology, Diagnostic and Veterinary Clinic Department, Sport Horse Research Centre, University of Perugia
Annalisa BIETTA
Department of Biopathological Science and Hygiene of Food and Animal Production, Faculty of Veterinary Medicine, University of Perugia
Maria Luisa MARENZONI
Pathology, Diagnostic and Veterinary Clinic Department, Sport Horse Research Centre, University of Perugia
Doreene R. HYATT
Department of Microbiology, Immunology, and Pathology, Colorado State University
Mauro COLETTI
Pathology, Diagnostic and Veterinary Clinic Department, Sport Horse Research Centre, University of Perugia
Fabrizio PASSAMONTI
Pathology, Diagnostic and Veterinary Clinic Department, Sport Horse Research Centre, University of Perugia

Keywords: bovine, PCR, staphylococcal enterotoxins, Staphylococcus aureus, virulence determinants

JOURNAL FREE ACCESS Advance online publication

Article ID: 10-0158

DOI https://doi.org/10.1292/jvms.10-0158

The final version of this article is now available: Vol. 72 (2010) , No. 11 p.1443

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Abstract

The aim of the study was to evaluate the genotypic characteristics of Staphylococcus aureus isolates (n=170) from bovine milk collected from seven dairy farms in Italy. On the basis of cultural and biochemical properties and by amplification of the 23S rRNA specific to S. aureus, all isolates were identified as S. aureus. To genotypically characterize S. aureus isolates, genes encoding virulence determinants (nuc, clfA, spa-IgG-binding, spa-X-region, fnbA and fnbB, cap5 and cap8) and staphylococcal enterotoxins (sea, seb, sec, sed, see, seg, seh, sei, sej) were investigated using PCR technique. The results showed that the isolates of S. aureus in each farm have the same genotypic characteristics, while the isolates genotipically differ between the different farms. The present study might help to understand the distribution of prevalent S. aureus strains in dairy farms.

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