Abstract
The objective of this study was to determine the effects of NEFA and glucose on carnitine palmitoyltransferase-I (CPT-I) mRNA expression, using real time reverse transcription polymerase chain reaction and ELISA methods, in cultured bovine hepatocytes. The results indicated that CPT-I transcription increased gradually, but CPT-I translation was not significantly changed, with glucose concentrations ranging from 0 to 3.0 mmol/l (P<0.01). And CPT-I transcription and translation were enhanced significantly when NEFA concentrations increased from 0 to 1.2 mmol/l and decreased significantly when NEFA concentrations increased from 1.2 to 4.8 mmol/l (P<0.01). High concentration NEFA was found to reduce fatty acids oxidation, potentially explaining the development from NEB to ketosis in dairy cows.
