Effects of Ginkgo biloba Extract on Promotion of Neurogenesis in the Hippocampal Dentate Gyrus in C57BL/6 Mice

Dae Young YOO; YoonYi NAM; Woosuk KIM; Ki-Yeon YOO; Jaeil PARK; Choong Hyun LEE; Jung Hoon CHOI; Yeo Sung YOON; Dong-Woo KIM; Moo-Ho WON; In Koo HWANG

doi:10.1292/jvms.10-0294

This article has now been updated. Please use the final version.

Effects of Ginkgo biloba Extract on Promotion of Neurogenesis in the Hippocampal Dentate Gyrus in C57BL/6 Mice

Dae Young YOO, YoonYi NAM, Woosuk KIM, Ki-Yeon YOO, Jaeil PARK, Choong Hyun LEE, Jung Hoon CHOI, Yeo Sung YOON, Dong-Woo KIM, Moo-Ho WON, In Koo HWANG

Author information

Dae Young YOO
Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University
YoonYi NAM
Institute of Natural Medicine, Hallym University
Woosuk KIM
Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University
Ki-Yeon YOO
Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University
Jaeil PARK
Central Research Center, Natural F&P Co., Ltd.
Choong Hyun LEE
Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University
Jung Hoon CHOI
Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University
Yeo Sung YOON
Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University
Dong-Woo KIM
Central Research Center, Natural F&P Co., Ltd.
Moo-Ho WON
Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University
In Koo HWANG
Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University

Keywords: Ginkgo biloba leaf extract, immunohistochemistry, neurogenesis, subgranular zone, western blot

JOURNAL FREE ACCESS Advance online publication

Article ID: 10-0294

DOI https://doi.org/10.1292/jvms.10-0294

The final version of this article is now available: Vol. 73 (2011) , No. 1 p.71

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Abstract

Ginkgo biloba leaf extract (Gb) has been known to improve blood flow and preclude the tissue from free radical damage. Regards, effects of Gb was examined by using Ki67, a specific proliferative marker for cellular proliferation, and doublecortin (DCX), indicating degree of neuroblast differentiation in the hippocampal dentate gyrus (DG) of adult C57BL/6 mice. The mice were fed with Gb at 40 and 100 mg/kg once daily for 28 days. The increase of Ki67- and DCX-immunoreactive cells in the DG was increased in a dose-dependent manner. Especially, the group having 100 mg/kg Gb showed a significant increase of DCX-immunoreactive neuroblasts with well-developed (tertiary) dendrites. Expression of DCX protein in the Gb groups was also significantly increased upon compared with the vehicle group. The results suggested that repeated intake of Gb would enhance cell proliferation and neuroblast differentiation in the mouse DG.

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