Construction of Swine-Specific CpG Motif Plasmid and the Study of Its Immunostimulatory Effects both In Vitro and In Vivo

Xiaoyu GUO; Hong JIA; Weifeng YUAN; Quan ZHANG; Shaohua HOU; Yao SUN; Gang ZHU; Hongfei ZHU; José Manuel SÁNCHEZ-VIZCAÍNO

doi:10.1292/jvms.11-0543

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Construction of Swine-Specific CpG Motif Plasmid and the Study of Its Immunostimulatory Effects both In Vitro and In Vivo

Xiaoyu GUO, Hong JIA, Weifeng YUAN, Quan ZHANG, Shaohua HOU, Yao SUN, Gang ZHU, Hongfei ZHU, José Manuel SÁNCHEZ-VIZCAÍNO

著者情報

Xiaoyu GUO
Institute of Animal Science , Chinese Academy of Agricultural Sciences
Centro de Vigilancia Sanitaria Veterinaria (VISAVET) and Animal Health Department, Complutense University of Madrid
Hong JIA
Institute of Animal Science , Chinese Academy of Agricultural Sciences
Weifeng YUAN
Institute of Animal Science , Chinese Academy of Agricultural Sciences
Quan ZHANG
College of Veterinary Medicine, Yangzhou University
Shaohua HOU
Institute of Animal Science , Chinese Academy of Agricultural Sciences
Yao SUN
China Animal Husbandry Group Research Institute
Gang ZHU
Xinjiang Tiankang Animal Science Bio-Technology Co. Ltd.
Hongfei ZHU
Institute of Animal Science , Chinese Academy of Agricultural Sciences
José Manuel SÁNCHEZ-VIZCAÍNO
Centro de Vigilancia Sanitaria Veterinaria (VISAVET) and Animal Health Department, Complutense University of Madrid

キーワード: antibody titer, CpG motif, FMD killed vaccine, lymphocyte transformation test, Stimulation Index

ジャーナルフリー早期公開

論文ID: 11-0543

DOI https://doi.org/10.1292/jvms.11-0543

この記事には本公開記事があります。

The final version of this article is now available: Vol. 74 (2012), No. 12

詳細

抄録

A swine-specific CpG motif enriched plasmid (pUC18-CpG) was constructed in this study. Its immunostimulant property was tested in vitro via lymphocyte transformation assay using swine peripheral blood mononuclear cells (PBMCs). The recombinant plasmid showed high Stimulation Index (SI) compared to the positive control (LPS). In a following animal vaccination-challenge experiment, pUC18-CpG was co-administered with a commercial swine FMD killed vaccine. Animals in the pUC18-CpG adjuvanted groups showed much higher antibody titers during the vaccination period.

責任著者(Corresponding author)

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