Development of a Sandwich Enzyme-Linked Immunosorbent Assay to Detect and Measure Serum Levels of Canine Ferritin

Seishiro CHIKAZAWA; Yasutomo HORI; Fumio HOSHI; Kazutaka KANAI; Naoyuki ITO; Jun SATO; Koichi ORINO; Kiyotaka WATANABE; Sei-ichi HIGUCHI

doi:10.1292/jvms.12-0324

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Development of a Sandwich Enzyme-Linked Immunosorbent Assay to Detect and Measure Serum Levels of Canine Ferritin

Seishiro CHIKAZAWA, Yasutomo HORI, Fumio HOSHI, Kazutaka KANAI, Naoyuki ITO, Jun SATO, Koichi ORINO, Kiyotaka WATANABE, Sei-ichi HIGUCHI

著者情報

Seishiro CHIKAZAWA
Department of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University
Yasutomo HORI
Department of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University
Fumio HOSHI
Department of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University
Kazutaka KANAI
Department of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University
Naoyuki ITO
Department of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University
Jun SATO
Tomizawa Animal Hospital
Koichi ORINO
Laboratory of Biochemistry, School of Veterinary Medicine, Kitasato University
Kiyotaka WATANABE
Laboratory of Biochemistry, School of Veterinary Medicine, Kitasato University
Sei-ichi HIGUCHI
Department of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University

キーワード: canine, ferritin, sandwich ELISA

ジャーナルフリー早期公開

論文ID: 12-0324

DOI https://doi.org/10.1292/jvms.12-0324

この記事には本公開記事があります。

The final version of this article is now available: Vol. 75 (2013), No. 4 p. 515

詳細

抄録

We established a homologous sandwich enzyme-linked immunosorbent assay (ELISA) to measure serum levels of canine ferritin. Our assay uses a rabbit anti-canine heart ferritin polyclonal antibody, and canine heart ferritin as a standard. Serum ferritin concentration in healthy dogs (n=163) was 789 ± 284 ng/ml (mean ± standard deviation), a value higher than reported previously. Confidence levels relating to repeatability, dilution, and recovery for this method were high. Therefore, we believe our developed sandwich ELISA will be effective in evaluating serum levels of canine ferritin.

責任著者(Corresponding author)

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