Canine Amino Acid Transport System Xc−: cDNA Sequence, Distribution and Cystine Transport Activity in Lens Epithelial Cells

Takuya MARUO; Nobuyuki KANEMAKI; Ken ONDA; Reiichiro SATO; Nobuteru ICHIHARA; Hideharu OCHIAI

doi:10.1292/jvms.13-0170

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Canine Amino Acid Transport System X_c⁻: cDNA Sequence, Distribution and Cystine Transport Activity in Lens Epithelial Cells

Takuya MARUO, Nobuyuki KANEMAKI, Ken ONDA, Reiichiro SATO, Nobuteru ICHIHARA, Hideharu OCHIAI

著者情報

キーワード: cysteine/glutamate exchanger, glutathione, lens epithelial cells, SLC7A11, system x_c⁻

ジャーナルフリー早期公開

論文ID: 13-0170

DOI https://doi.org/10.1292/jvms.13-0170

この記事には本公開記事があります。

The final version of this article is now available: Vol. 76 (2014), No. 4 pp. 523-530

詳細

抄録

The cystine transport activity of a lens epithelial cell line originated from a canine mature cataract was investigated. The distinct cystine transport activity was observed, which was inhibited to 28% by extracellular 1mM glutamate. The cDNA sequences of canine cysteine/glutamate exchanger (xCT) and 4F2hc were determined. The predicted amino acid sequences were 527 and 533 amino acid polypeptides, respectively. The amino acid sequences of canine xCT and 4F2hc showed high similarities (>80%) to those of humans. The expression of xCT in lens epithelial cell line was confirmed by western blot analysis. RT-PCR analysis revealed high level expression only in the brain, and it was below the detectable level in other tissues.

責任著者(Corresponding author)

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