Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250

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Effect of Freezing Treatment on Colostrum to Prevent the Transmission of Bovine Leukemia Virus
Toru KANNORyoko ISHIHARAShinichi HATAMAYasuhiro OUEHiroki EDAMATSUYasuhiro KONNOSatoshi TACHIBANAKenji MURAKAMI
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JOURNAL FREE ACCESS Advance online publication

Article ID: 13-0253

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Abstract
Here, we used a sheep bioassay to determine the effect of freezing colostrum to prevent the transmission of bovine leukemia virus (BLV) among neonatal calves. Leukocytes were isolated from the colostrum of a BLV-infected Holstein cow, which were then either left untreated (control) or freeze-thawed. A sheep inoculated intraperitoneally with the untreated leukocytes were infected with BLV at 3 weeks after inoculation, whereas the sheep inoculated with treated leukocytes did not become infected. The uninfected sheep was inoculated again with leukocytes isolated from the colostrum of another BLV-infected Holstein cow after freezing treatment, and again did not become infected with BLV. Finally, this sheep was inoculated with the leukocytes isolated from the colostrum of another virus-infected cow without freezing treatment, and became infected with BLV at 4 weeks after inoculation. The results indicate that colostrum should be frozen as a useful means of inactivating the infectivity of BLV-infected lymphocytes.
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© 2013 by the Japanese Society of Veterinary Science

この記事はクリエイティブ・コモンズ [表示 - 非営利 - 改変禁止 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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