High Genetic Diversity of Anaplasma marginale Detected from Philippine Cattle

Adrian Patalinghug YBAÑEZ; Rochelle Haidee D. YBAÑEZ; Florencia G. CLAVERIA; Mary Jane CRUZ-FLORES; Xuen XUENAN; Naoaki YOKOYAMA; Hisashi INOKUMA

doi:10.1292/jvms.13-0405

This article has now been updated. Please use the final version.

High Genetic Diversity of Anaplasma marginale Detected from Philippine Cattle

Adrian Patalinghug YBAÑEZ, Rochelle Haidee D. YBAÑEZ, Florencia G. CLAVERIA, Mary Jane CRUZ-FLORES, Xuen XUENAN, Naoaki YOKOYAMA, Hisashi INOKUMA

Author information

Adrian Patalinghug YBAÑEZ
Department of Clinical Veterinary Sciences, Obihiro University of Agriculture and Veterinary Medicine
United Graduate School of Veterinary Sciences, Gifu University
Visayas State University
Rochelle Haidee D. YBAÑEZ
Visayas State University
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
Florencia G. CLAVERIA
Biology Department, College of Science, De La Salle University-Manila
Mary Jane CRUZ-FLORES
Biology Department, College of Science, De La Salle University-Manila
Xuen XUENAN
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
Naoaki YOKOYAMA
United Graduate School of Veterinary Sciences, Gifu University
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
Hisashi INOKUMA
Department of Clinical Veterinary Sciences, Obihiro University of Agriculture and Veterinary Medicine
United Graduate School of Veterinary Sciences, Gifu University

Keywords: Anaplasma marginale, cattle, groEL, Msp1a, Philippines

JOURNAL FREE ACCESS Advance online publication

Article ID: 13-0405

DOI https://doi.org/10.1292/jvms.13-0405

The final version of this article is now available: Vol. 76 (2014), No. 7 pp. 1009-1014

Details

Abstract

A total of 658 cattle in 6 provinces in the Philippines were screened for Anaplasma marginale infection by using a diagnostic heat-shock operon (groEL) gene-PCR assay. The screening-positive samples were further tested using the major surface antigen protein 1a (Msp1a) gene-PCR assay. Screening PCR results showed 130 cattle (19.8%) were positive for the A. marginale infection. Subsequent amplification using the Msp1a gene only showed 93 samples (14.1%) to be positive. In addition, 37 tandem-repeat structures, including 20 novel structures, and 41 distinct genotypes were identified. Interestingly, multiple infections of 4 different genotypes were also observed in A. marginale-infected cattle. The present study demonstrated the prevalence and characterization of diverse genotypes of A. marginale in the Philippine cattle.

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