Analysis of the Mechanism of Reactivation of Latently Infecting Pseudorabies Virus by Acetylcholine

Abstract

In this study, the effect of cholinergic or adrenergic inhibitors on the reactivation of latent Pseudorabies virus (PRV) was analyzed to clarify the mechanism of the reactivation of latent PRV by acetylcholine. For acetylcholine inhibition, latently infected mice were injected with scopolamine or succynilcholine before acetylcholine stimulation. For sympathetic blocking, mice were preinjected intraperitoneally with phenoxybenzamine or propranolol. The signals to both acetylcholine receptors had no relationship to the reactivation of latent PRV, and both sympathetic blockers showed inhibition of PRV reactivation by acetylcholine. In our reactivation model, a large amount of acetylcholine may stimulate the sympathetic nerve system in some way to reactivate the virus.