High-Molecular-Weight Polyethylene Glycol Enhances Hypothermic Storage of Feline Kidney Cells

Masaaki KATAYAMA; Shinobu TSUCHIAKA; Tomoki MOTEGI; Masao MIYAZAKI; Tetsuro YAMASHITA; Shunsuke SHIMAMURA; Yasuhiko OKAMURA; Yuji UZUKA

doi:10.1292/jvms.13-0565

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High-Molecular-Weight Polyethylene Glycol Enhances Hypothermic Storage of Feline Kidney Cells

Masaaki KATAYAMA, Shinobu TSUCHIAKA, Tomoki MOTEGI, Masao MIYAZAKI, Tetsuro YAMASHITA, Shunsuke SHIMAMURA, Yasuhiko OKAMURA, Yuji UZUKA

著者情報

キーワード: cold storage, feline kidney transplantation, kidney cells, polyethylene glycol

ジャーナルフリー早期公開

論文ID: 13-0565

DOI https://doi.org/10.1292/jvms.13-0565

この記事には本公開記事があります。

The final version of this article is now available: Vol. 76 (2014), No. 6 pp. 923-926

詳細

抄録

Phosphate-buffered sucrose (PBSc) solution is effective for short-term hypothermic preservation of tissue during feline kidney transplantation. A high-molecular-weight polyethylene glycol (35,000 Da, PEG35) reportedly enhanced the protective effects against cold-induced tubular injuries in animal kidney transplantation models. We investigated the ability of PBSc solution containing PEG35 to preserve cultured feline kidney cells using in vitro WST-8 cell proliferation assays. PEG35 significantly improved cell viability during 24 hr of cold preservation. PBSc containing 20 g/l PEG35 achieved an effect almost equal to that of University of Wisconsin (UW) solution, the gold standard preservation solution used in human clinical kidney transplantation, for up to 24 hr of preservation. Our results suggest that PBSc containing PEG35 provides an excellent medium for graft cold storage during feline kidney transplantation.

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