Endostatin inhibits T-type Ca²⁺ channel current in guinea pig ventricular myocyte

Abstract

Endostatin, a fragment of collagen XVIII, is known as an endogenous angiogenesis inhibitor, and its serum concentration increases in various cardiovascular diseases. T-type Ca²⁺ channel, low voltage-activated Ca²⁺ channel, is not expressed in adult ventricular myocytes. Re-expression of T-type Ca²⁺ channels in cardiac myocytes is thought to be involved in the development of cardiac hypertrophy. We examined the effects of endostatin on T-type Ca²⁺ channel current by whole-cell patch clamp technique in freshly isolated adult guinea pig ventricular myocytes, which exceptionally express T-type Ca²⁺ channels. Although endostatin 300 ng/ml had no effect on L-type Ca²⁺ current, it significantly inhibited T-type Ca²⁺ current. These data indicate that endostatin can be an endogenous inhibitor of T-type Ca²⁺ channels in the cardiac myocytes.