Ubiquitin-specific protease 9X in host cells interacts with herpes simplex virus 1 ICP0

Yuka SATO; Akihisa KATO; Jun ARII; Naoto KOYANAGI; Hiroko KOZUKA-HATA; Masaaki OYAMA; Yasushi KAWAGUCHI

doi:10.1292/jvms.15-0598

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Ubiquitin-specific protease 9X in host cells interacts with herpes simplex virus 1 ICP0

Yuka SATO, Akihisa KATO, Jun ARII, Naoto KOYANAGI, Hiroko KOZUKA-HATA, Masaaki OYAMA, Yasushi KAWAGUCHI

著者情報

Yuka SATO
Division of Molecular Virology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo
Department of Infectious Disease Control, International Research Center for Infectious Diseases, The Institute of Medical Science, The University of Tokyo
Akihisa KATO
Division of Molecular Virology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo
Department of Infectious Disease Control, International Research Center for Infectious Diseases, The Institute of Medical Science, The University of Tokyo
Jun ARII
Division of Molecular Virology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo
Department of Infectious Disease Control, International Research Center for Infectious Diseases, The Institute of Medical Science, The University of Tokyo
Naoto KOYANAGI
Division of Molecular Virology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo
Department of Infectious Disease Control, International Research Center for Infectious Diseases, The Institute of Medical Science, The University of Tokyo
Hiroko KOZUKA-HATA
Medical Proteomics Laboratory, The Institute of Medical Science, The University of Tokyo
Masaaki OYAMA
Medical Proteomics Laboratory, The Institute of Medical Science, The University of Tokyo
Yasushi KAWAGUCHI
Division of Molecular Virology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo
Department of Infectious Disease Control, International Research Center for Infectious Diseases, The Institute of Medical Science, The University of Tokyo

キーワード: deubiquitylating enzyme, E3 ubiquitin ligase, herpes simplex virus, ICP0, USP9X

ジャーナルフリー早期公開

論文ID: 15-0598

DOI https://doi.org/10.1292/jvms.15-0598

この記事には本公開記事があります。

The final version of this article is now available: Vol. 78 (2016), No. 3 pp. 405-410

詳細

抄録

Herpes simplex virus 1 (HSV-1) expresses infected cell protein 0 (ICP0), a multi-functional protein with E3 ubiquitin ligase activity and a critical regulator of the viral life cycle. To obtain novel insights into the molecular mechanism by which ICP0 regulates HSV-1 replication, we analyzed HEp-2 cells infected with HSV-1 by tandem affinity purification and mass spectrometry-based proteomics. This screen identified 50 host-cell proteins that potentially interact with ICP0, including ubiquitin-specific protease 9X (USP9X). The interaction between ICP0 and USP9X was confirmed by co-immunoprecipitation. Notably, USP9X depletion increased the ICP0 abundance and promoted viral replication. These results suggest that USP9X-dependent regulation of ICP0 expression is part of a complex feedback mechanism that facilitates optimal HSV-1 replication.

責任著者(Corresponding author)

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