Molecular cloning, phylogenetic analysis and heat shock response of Babesia gibsoni heat shock protein 90

Masahiro YAMASAKI; Yoshihiro TSUBOI; Yusuke TANIYAMA; Naohiro UCHIDA; Reeko SATO; Kensuke NAKAMURA; Hiroshi OHTA; Mitsuyoshi TAKIGUCHI

doi:10.1292/jvms.16-0027

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Molecular cloning, phylogenetic analysis and heat shock response of Babesia gibsoni heat shock protein 90

Masahiro YAMASAKI, Yoshihiro TSUBOI, Yusuke TANIYAMA, Naohiro UCHIDA, Reeko SATO, Kensuke NAKAMURA, Hiroshi OHTA, Mitsuyoshi TAKIGUCHI

著者情報

キーワード: Babesia gibsoni, heat shock protein 90, phylogenetic analysis

ジャーナルフリー早期公開

論文ID: 16-0027

DOI https://doi.org/10.1292/jvms.16-0027

この記事には本公開記事があります。

The final version of this article is now available: Vol. 78 (2016), No. 8 pp. 1355-1360

詳細

抄録

The Babesia gibsoni heat shock protein 90 (BgHSP90) gene was cloned and sequenced. The length of the gene was 2,610 bp with two introns. This gene was amplified from cDNA corresponding to full length cording sequence (CDS) with an open reading frame of 2,148 bp. A phylogenetic analysis of the CDS of HSP90 gene showed that B. gibsoni was most closely related to B. bovis and Babesia sp. BQ1/Lintan and lies within a phylogenetic cluster of protozoa. Moreover, mRNA transcription profile for BgHSP90 exposed to high temperature were examined by quantitative real-time reverse transcription-polymerase chain reaction. BgHSP90 levels was elevated when the parasites were incubated at 43°C for 1 hr.

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