Pathogenesis of the attenuated foot-and-mouth disease virus O/JPN/2000 in experimentally infected pigs

Manabu YAMADA; Katsuhiko FUKAI; Kazuki MORIOKA; Tatsuya NISHI; Reiko YAMAZOE; Rie KITANO; Nobuaki SHIMADA; Kazuo YOSHIDA; Toru KANNO; Kenichi SAKAMOTO; Makoto YAMAKAWA

doi:10.1292/jvms.18-0377

この記事には本公開記事があります。本公開記事を参照してください。
引用する場合も本公開記事を引用してください。

Pathogenesis of the attenuated foot-and-mouth disease virus O/JPN/2000 in experimentally infected pigs

Manabu YAMADA, Katsuhiko FUKAI, Kazuki MORIOKA, Tatsuya NISHI, Reiko YAMAZOE, Rie KITANO, Nobuaki SHIMADA, Kazuo YOSHIDA, Toru KANNO, Kenichi SAKAMOTO, Makoto YAMAKAWA

著者情報

Manabu YAMADA
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization
Katsuhiko FUKAI
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization
Kazuki MORIOKA
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization
Tatsuya NISHI
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization
Reiko YAMAZOE
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization
Rie KITANO
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization
Nobuaki SHIMADA
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization
Kazuo YOSHIDA
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization
Toru KANNO
Hokkaido Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization
Kenichi SAKAMOTO
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization
Makoto YAMAKAWA
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization

キーワード: foot-and-mouth disease virus, histopathology, immunohistochemistry, pig

ジャーナルフリー早期公開

論文ID: 18-0377

DOI https://doi.org/10.1292/jvms.18-0377

この記事には本公開記事があります。

The final version of this article is now available: Vol. 80 (2018), No. 11 pp. 1669-1675

詳細

抄録

We examined the pathogenesis of the attenuated foot-and-mouth disease virus (FMDV) O/JPN/2000 in pigs. The virus used in this study was passaged three times in primary bovine kidney (BK) cells and once in baby hamster kidney-21 (BHK-21) cells after isolation. A plaque assay demonstrated that this virus exhibited the small plaque (SP) phenotype. There was no clinical or histological evidence of vesicular lesions in pigs intraorally inoculated with 10⁶ 50% tissue culture infectious dose (TCID₅₀)/ml of the SP virus (SPV) of FMDV O/JPN/2000. Although fever was detected from 2 or 3 days post inoculation (dpi), there was no other prominent clinical sign up to 6 dpi. Virus shedding from saliva and nasal swab samples was not observed in any pigs inoculated with the SPV of FMDV O/JPN/2000. In the foot, mild lamellar degeneration of prickle cells in the upper layer of the stratum spinosum was histologically observed without development into vesicular or necrotic lesions. Immunohistochemical virus antigen- and terminal deoxynucleotidyl transferase-mediated dUTP-nick end labeling (TUNEL)-positive reactions observed in the foot at 1 dpi seemed to disappear after 3 and 6 dpi. Our findings suggest that the SPV of FMDV O/JPN/2000 had low pathogenicity against pigs by intraoral inoculation.

責任著者(Corresponding author)

J-STAGEへの登録はこちら（無料）