Phylogenetic position of Nyctotherus teleacus isolated from a tortoise (Astrochelys radiata) and its electron microscopic features

Jun SUZUKI; Seiki KOBAYASHI; Naoko YOSHIDA; Yoshiyuki AZUMA; Namiko KOBAYASHI-OGATA; Dwi Peni KARTIKASARI; Yasuaki YANAGAWA; Satoshi IWATA

doi:10.1292/jvms.20-0004

This article has now been updated. Please use the final version.

Phylogenetic position of Nyctotherus teleacus isolated from a tortoise (Astrochelys radiata) and its electron microscopic features

Jun SUZUKI, Seiki KOBAYASHI, Naoko YOSHIDA, Yoshiyuki AZUMA, Namiko KOBAYASHI-OGATA, Dwi Peni KARTIKASARI, Yasuaki YANAGAWA, Satoshi IWATA

Author information

Jun SUZUKI
Division of Food Microbiology, Department of Microbiology, Tokyo Metropolitan Institute of Public Health
Seiki KOBAYASHI
Department of Infectious Diseases, Keio University School of Medicine
Naoko YOSHIDA
Department of Infectious Diseases, Keio University School of Medicine
Yoshiyuki AZUMA
Laboratory of Animal and Human Nutritional Physiology, Kitasato University School of Veterinary Medicine
Namiko KOBAYASHI-OGATA
Center for Advanced Marine Core Research, Kochi University
Dwi Peni KARTIKASARI
Department of Parasitology, Faculty of Medicine, Universitas Airlangga, Kampus A UNAIR
Yasuaki YANAGAWA
AIDS Clinical Center, National Center for Global Health and Medicine
Satoshi IWATA
Department of Infectious Diseases, Keio University School of Medicine

Keywords: Astrochelys radiate, electron microscopic feature, genetic phylogeny, Nyctotheroides sp. , Nyctotherus teleacus

JOURNAL OPEN ACCESS Advance online publication

Article ID: 20-0004

DOI https://doi.org/10.1292/jvms.20-0004

The final version of this article is now available: Vol. 82 (2020), No. 6 pp. 699-703

Details

Abstract

A commensal ciliate was isolated from the stool of a tortoise (Astrochelys radiata). The ciliate was classified as Nyctotherus teleacus, according to its basic morphological features. Electron microscopic observations using cultured N. teleacus (NictoT1 strain) revealed many spherical hydrogenosomes and methanogen-suspected bacteria, together with a characteristic triangular macronucleus containing many spherical chromosomes in the cytoplasm of NictoT1. The results of phylogenetic analysis showed that NictoT1 was included in the cluster of Nyctotheroides spp. (family Nyctotheridae). Nyctotheroides spp. commonly infest amphibians, which are taxonomically closely related to reptiles, including the tortoises evaluated in the present study.

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